beta-1,4-Galactosyltransferase 1 (Y285L)  (Synonyms: B4GALT1 (Y285L))

beta-1,4-Galactosyltransferase 1 (Y285L) can enzymatic synthesis of the LacdiNAc motif. beta-1,4-Galactosyltransferase 1 (Y285L) can transfer of GalNAc from UDP-GalNAc^[1].

Product Information
This product is expressed in recombinant HEK293 cells, C-terminal His Tagged, molecular weight 39.7 kDa, storage buffer components are 20 mM Tris-HCl (PH7.4), 200 mM NaCl, 20% Glycerin, can effectively transfer GalNAc or GalNAz and other derivatives to the terminal GlcNAc residue, the latter can be used in antibody-drug conjugates (ADC) as targeted cancer therapy, etc.

Instructions (can be adjusted according to experimental conditions)
1. Reaction system:
100 mM Tris-HCl (pH 7.5), 6 mM MnCl2;
2. Reaction reagents:
1) UDP-GalNAz: antibody (equivalent ratio) = 300:1;
2) Enzyme addition amount: enzyme/antibody = 5%;
3) Prepare the reaction solution according to the above conditions, react at 37℃ for 48 hours; then place at -15℃ to -25℃ to stop the reaction and perform sample testing.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Liquid

Colorless to light yellow

[beta-1,4-Galactosyltransferase 1 (Y285L)]

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Ran Cao, et al. Complete Shift in Glycosyl Donor Specificity in Mammalian, but not C. elegans β1,4-GalT1 Y286L mutants, Enables the Synthesis of N,N-Diacetyllactosamine. Chemistry Europe. 2022.