Beta-Sitosterol (purity>75%) (Synonyms: β-Sitosterol (purity>75%); 22,23-Dihydrostigmasterol (purity>75%))

Name: Beta-Sitosterol (purity>75%)
Brand: MedChemExpress
SKU: HY-N0171B
Rating: 4.5 (1 reviews)

Cat. No.: HY-N0171B Purity: ≥98.0%: FAQs
Data Sheet SDS COA Handling Instructions

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Beta-Sitosterol (purity>75%) is a phytosterol with oral activity. Beta-Sitosterol (purity>75%) interferes with a variety of cell signaling pathways, including the cell cycle, apoptosis and cell proliferation. Beta-Sitosterol (purity>75%) has anti-inflammatory, antioxidant, and antitumor activities.

For research use only. We do not sell to patients.

Beta-Sitosterol (purity>75%) Chemical Structure

CAS No. : 83-46-5

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10 mg	USD 60	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 110	In-stock	Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Description

In Vitro

Beta-Sitosterol purity>75% (16 μM, 1, 3, 5 days) can inhibit the growth of MDA-MB-231 human breast cancer cells and induce cell apoptosis^[1].
Beta-Sitosterol purity>75% (120, 240 μM, 24 h) shows significant dose-dependent growth inhibition on COLO 320 DM cells (IC₅₀ 266.2 μM). The expression of β-catenin and PCNA antigens in human colon cancer cells was inhibited by scavenging reactive oxygen species to induce cell apoptosis^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay^[1]

Cell Line:	MDA-MB-231
Concentration:	16 μM
Incubation Time:	3 days
Result:	No cytotoxicity at 16 μM

Apoptosis Analysis^[1]

Cell Line:	MDA-MB-231
Concentration:	16 μM
Incubation Time:	5 days
Result:	Increased 33% apoptosis when assayed using 1×10⁴ cells and a 6-fold increase in apoptosis when assayed using a smaller number of cells 5×10³.

Western Blot Analysis^[2]

Cell Line:	COLO 320 DM
Concentration:	15, 30, 60, 120 μM
Incubation Time:	24 h
Result:	Decreased β-catenin and PCNA expression.

In Vivo

Beta-Sitosterol purity>75% (10-20 mg/kg, suspended in 0.1% CMC, orally, once a day for 16 weeks) can prevent cancer in the rat model of colon cancer^[2].
Beta-Sitosterol purity>75% (10, 15, 20 mg/kg, orally, for 21 consecutive days) has anti-hyperglycemic and antioxidant effects in streptozotocin (HY-13753) induced experimental hyperglycemic rat models^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Colon cancer rats model ^[2]
Dosage:	10, 20 mg/kg
Administration:	p.o., suspended in 0.1% CMC (1.0 mL)
Result:	Reduced the number of aberrant crypt and crypt multiplicity in a dose-dependent manner.

Animal Model:	Streptozotocin-induced hyperglycemia rats model^[3]
Dosage:	10, 15, 20 mg/kg
Administration:	p.o.
Result:	Increased insulin levels and decreased HbA1c levels. Improved pancreatic antioxidant levels and decreased LPO levels.

Molecular Weight

414.71

Formula

C₂₉H₅₀O

CAS No.

83-46-5

Appearance

Solid

Color

White to off-white

SMILES

CC[C@@H](C(C)C)CC[C@@H](C)[C@H]1CC[C@@]2([H])[C@]3([H])CC=C4C[C@@H](O)CC[C@]4(C)[C@@]3([H])CC[C@]12C

Structure Classification

Steroids

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMF : 16.67 mg/mL (40.20 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.4113 mL	12.0566 mL	24.1132 mL
5 mM	0.4823 mL	2.4113 mL	4.8226 mL
10 mM	0.2411 mL	1.2057 mL	2.4113 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMF 90% Corn Oil
Solubility: ≥ 1.67 mg/mL (4.03 mM); Clear solution

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: ≥98.0%

Select Batch:

Data Sheet (277 KB) SDS (393 KB)

COA (251 KB) HNMR (263 KB)

Handling Instructions (2659 KB)

References

[1]. Awad AB, et al. Inhibition of growth and stimulation of apoptosis by beta-sitosterol treatment of MDA-MB-231 human breast cancer cells in culture. Int J Mol Med. 2000 May;5(5):541-5. [Content Brief]

[2]. Baskar AA, et al. Chemopreventive potential of beta-Sitosterol in experimental colon cancer model--an in vitro and In vivo study. BMC Complement Altern Med. 2010 Jun 4;10:24. [Content Brief]

[3]. Gupta R, et al. Antidiabetic and antioxidant potential of β-sitosterol in streptozotocin-induced experimental hyperglycemia. J Diabetes. 2011 Mar;3(1):29-37. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMF	1 mM	2.4113 mL	12.0566 mL	24.1132 mL	60.2831 mL
	5 mM	0.4823 mL	2.4113 mL	4.8226 mL	12.0566 mL
	10 mM	0.2411 mL	1.2057 mL	2.4113 mL	6.0283 mL
	15 mM	0.1608 mL	0.8038 mL	1.6075 mL	4.0189 mL
	20 mM	0.1206 mL	0.6028 mL	1.2057 mL	3.0142 mL
	25 mM	0.0965 mL	0.4823 mL	0.9645 mL	2.4113 mL
	30 mM	0.0804 mL	0.4019 mL	0.8038 mL	2.0094 mL
	40 mM	0.0603 mL	0.3014 mL	0.6028 mL	1.5071 mL

Beta-Sitosterol (purity>75%) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Beta-Sitosterol (purity>75%)83-46-5β-Sitosterol (purity>75%) 22,23-Dihydrostigmasterol (purity>75%)ApoptosisMDA-MB-231COLO 320 DMColon cancer rats modelStreptozotocin-induced hyperglycemiaInhibitorinhibitorinhibit

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Beta-Sitosterol (purity>75%) (Synonyms: β-Sitosterol (purity>75%); 22,23-Dihydrostigmasterol (purity>75%))

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Complete Stock Solution Preparation Table

Beta-Sitosterol (purity>75%) Related Classifications

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