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Bicine is a buffer used in physiological research. Bicine is a derivative of the simple amino acid glycine that increases the sensitivity of guanosine enzyme histochemical detection.

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Bicine Chemical Structure

Bicine Chemical Structure

CAS No. : 150-25-4

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Based on 1 publication(s) in Google Scholar

Bicine Related Antibodies

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Description

Bicine is a buffer used in physiological research. Bicine is a derivative of the simple amino acid glycine that increases the sensitivity of guanosine enzyme histochemical detection[1][2].

In Vitro

Preparation of sections for histochemistry of guanine oxidase in human tissues[2]:
1. Tissue samples were snap frozen in liquid nitrogen and 10 µM thick cryostat sections were prepared.
2. After fixation with 2.5% glutaraldehyde in 0.05 M sodium cacodylate buffer (pH 7.4) at room temperature for 1 hour, sections were rinsed in Bicine buffer (0.1 M, pH 7.8) and incubated with xanthine oxidase at 37°C for 15 minutes, rinsed again in Bicine buffer and incubated at 37°C for 1-2 hours.
3. After rinsing in distilled water, sections were allowed to air dry, dehydrated with graded ethanol, transferred to xylene and mounted in synthetic resin.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Bicine Related Antibodies
Molecular Weight

163.17

Formula

C6H13NO4
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(O)CN(CCO)CCO
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Store at room temperature 3 years

In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

H2O : 166.67 mg/mL (1021.45 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 6.1286 mL 30.6429 mL 61.2858 mL
5 mM 1.2257 mL 6.1286 mL 12.2572 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 50 mg/mL (306.43 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: ≥98.0%

SDS (393 KB)

COA (244 KB) HNMR (224 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 6.1286 mL 30.6429 mL 61.2858 mL 153.2144 mL
5 mM 1.2257 mL 6.1286 mL 12.2572 mL 30.6429 mL
10 mM 0.6129 mL 3.0643 mL 6.1286 mL 15.3214 mL
15 mM 0.4086 mL 2.0429 mL 4.0857 mL 10.2143 mL
20 mM 0.3064 mL 1.5321 mL 3.0643 mL 7.6607 mL
25 mM 0.2451 mL 1.2257 mL 2.4514 mL 6.1286 mL
30 mM 0.2043 mL 1.0214 mL 2.0429 mL 5.1071 mL
40 mM 0.1532 mL 0.7661 mL 1.5321 mL 3.8304 mL
50 mM 0.1226 mL 0.6129 mL 1.2257 mL 3.0643 mL
60 mM 0.1021 mL 0.5107 mL 1.0214 mL 2.5536 mL
80 mM 0.0766 mL 0.3830 mL 0.7661 mL 1.9152 mL
100 mM 0.0613 mL 0.3064 mL 0.6129 mL 1.5321 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Bicine Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Bicine150-25-4Biochemical Assay Reagentsuseful bufferInhibitorinhibitorinhibit

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