Fluorescence Correlation Spectroscopy

Definition:

FCS monitors the random motion of fluorescently labelled molecules inside a defined volume irradiated by a focused laser beam. These fluctuations provide information on the rate of diffusion or diffusion time of a particle and this is directly dependent on the particle mass. As a consequence, any increase in the mass of a biomolecule, e.g. as a result of an interaction with a second molecule, is readily detected as an increase in the diffusion time of the particle. From these results the concentration of the different molecules can be calculated as well as their binding constant.

References:

[1]. Ontobee, Molecular Interactions Controlled Vocabulary, MI_0052, Fluorescence Correlation Spectroscopy.

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