Interactome Parallel Affinity Capture

Definition:

Interactome Parallel Affinity Capture is a specific protein-protein interaction capture method that uses bait proteins with at least two different affinity tags (such as GFP, FLAG, etc.), expressed endogenously and purified. Compared to Tandem Affinity Purification (TAP), this method employs parallel purification protocols, enhancing the specificity for capturing target proteins interacting with the bait proteins. This technique is primarily applied in systems biology and proteomics to identify and validate protein complexes and their dynamic changes.

References:

[1]. Rees JS, et al. In vivo analysis of proteomes and interactomes using Parallel Affinity Capture (iPAC) coupled to mass spectrometry. Mol Cell Proteomics. 2011 Jun;10(6):M110.002386. [Content Brief]

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