Biotin-16-dUTP trisodium  (Synonyms: Biotin-16-deoxyuridine-5'-triphosphate trisodium)

Biotin-16- dUTP (Biotin-16-deoxyuridine-5'-triphosphate) trisodium can be used to replace its natural counterpart dTTP by enzymatically incorporating it into DNA/cDNA. Biotin-16- dUTP trisodium can be used to produce biotinylated DNA probes in a variety of assay applications^[1][2].

Biotin-16- dUTP trisodium can be used for in situ detection of DNA fragments by nick end labelling (TUNEL assay) for the study of apoptosis^[1].
Biotin-16-dUTP trisodium can be used for replication foci labelling in permeabilised HeLa cell nuclei and detected by fluorescent streptavidin^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

1013.72

C₃₂H₄₉N₇Na₃O₁₈P₃S

Liquid

Colorless to light yellow

O=C(NC1=O)N([C@]2([H])O[C@@H]([C@H](C2)O)COP(OP(OP(O)(O[Na])=O)(O[Na])=O)(O[Na])=O)C=C1/C=C/CNC(CCCCCNC(CCCNC(CCCC[C@H]3[C@]4([H])[C@](NC(N4)=O)([H])CS3)=O)=O)=O

Room temperature in continental US; may vary elsewhere.

Solution, -20°C, protect from light, 2 years

• [1]. C Charriaut-Marlangue, et al. A cautionary note on the use of the TUNEL stain to determine apoptosis. Neuroreport. 1995 Dec 29;7(1):61-4.  [Content Brief]

  [2]. T Krude, et al. Chromatin assembly factor 1 (CAF-1) colocalizes with replication foci in HeLa cell nuclei. Exp Cell Res. 1995 Oct;220(2):304-11.  [Content Brief]