Biotin TSA（200×）  (Synonyms: Biotin Tyramide)

Biotin TSA (200×) (Biotin Tyramide) is a biotin derivative used for tyramide signal amplification (TSA), as a reagent to amplify both immunohistochemical signals and in situ hybridization protocols. Biotinyl tyramide can be used for the research of tyramide signal amplification^{[1][2][3][4][5]}.

Biotinyl tyramide (100 μM, 5min) and hemin (HY-19424) enhance biotinylation of starved U2OS cells after validating the G4-specific biotinylation activity of RNA-hemin complexes in vitro^[6].
Biotinyl tyramide (27.5 μM, 5min) binds to the tyrosine side chains of cell surface proteins in HaCaT wild-type cells or CRISPR-modified dual oxidase 1 (DUOX1) knockout cells^[7].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

[Biotin TSA（200×）]

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Evans M F, et al. Optimization of biotinyl-tyramide-based in situ hybridization for sensitive background-free applications on formalin-fixed, paraffin-embedded tissue specimens [J]. BMC Clinical Pathology, 2003, 3: 1-17.  [Content Brief]

  [2]. Kharel P, et al. Stress promotes RNA G-quadruplex folding in human cells [J]. Nature Communications, 2023, 14(1): 205.  [Content Brief]

  [3]. Bobrow M N, et al. Catalyzed reporter deposition, a novel method of signal amplification application to immunoassays [J]. Journal of immunological methods, 1989, 125(1-2): 279-285.  [Content Brief]

  [4]. Pató A, et al. Hydrogen peroxide production by epidermal dual oxidase 1 regulates nociceptive sensory signals [J]. Redox Biology, 2023, 62: 102670.  [Content Brief]

  [5]. Kim S H, et al. An improved protocol of biotinylated tyramine-based immunohistochemistry minimizing nonspecific background staining [J]. Journal of Histochemistry & Cytochemistry, 2003, 51(1): 129-132.  [Content Brief]

  [6]. Dráberová E, et al. Quantification of α-tubulin isotypes by sandwich ELISA with signal amplification through biotinyl-tyramide or immuno-PCR [J]. Journal of immunological methods, 2013, 395(1-2): 63-70.  [Content Brief]

  [7]. Hunyady B, et al. Immunohistochemical signal amplification by catalyzed reporter deposition and its application in double immunostaining [J]. Journal of Histochemistry & Cytochemistry, 1996, 44(12): 1353-1362.  [Content Brief]