Biotinyl tyramide

Name: Biotinyl tyramide
Brand: MedChemExpress
SKU: HY-125658
Rating: 5.0 (4 reviews)

Cat. No.: HY-125658 Purity: 99.80%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Biotinyl tyramide is a biotin derivative used for tyramide signal amplification (TSA), as a reagent to amplify both immunohistochemical signals and in situ hybridization protocols. Biotinyl tyramide can be used for the research of tyramide signal amplification.

For research use only. We do not sell to patients.

Biotinyl tyramide Chemical Structure

CAS No. : 41994-02-9

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 55	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 55	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 32	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 100	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 160	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 4 publication(s) in Google Scholar

Other Forms of Biotinyl tyramide:

Biotin TSA（200×） Get quote

4 Publications Citing Use of MCE Biotinyl tyramide

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

Biotinyl tyramide (100 μM, 5min) and hemin (HY-19424) enhance biotinylation of starved U2OS cells after validating the G4-specific biotinylation activity of RNA-hemin complexes in vitro^[6].
Biotinyl tyramide (27.5 μM, 5min) binds to the tyrosine side chains of cell surface proteins in HaCaT wild-type cells or CRISPR-modified dual oxidase 1 (DUOX1) knockout cells^[7].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

363.47

Formula

C₁₈H₂₅N₃O₃S

CAS No.

41994-02-9

Appearance

Solid

Color

White to off-white

SMILES

O=C(NCCC1=CC=C(O)C=C1)CCCC[C@@H]2SC[C@]([C@]2([H])N3)([H])NC3=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (275.13 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.7513 mL	13.7563 mL	27.5126 mL
5 mM	0.5503 mL	2.7513 mL	5.5025 mL
10 mM	0.2751 mL	1.3756 mL	2.7513 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (5.72 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (5.72 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (5.72 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.80%

Select Batch:

Data Sheet (278 KB) SDS (393 KB)

COA (245 KB) HNMR (144 KB) LCMS (85 KB)

Handling Instructions (2659 KB)

References

[1]. Evans M F, et al. Optimization of biotinyl-tyramide-based in situ hybridization for sensitive background-free applications on formalin-fixed, paraffin-embedded tissue specimens [J]. BMC Clinical Pathology, 2003, 3: 1-17. [Content Brief]

[2]. Bobrow M N, et al. Catalyzed reporter deposition, a novel method of signal amplification application to immunoassays [J]. Journal of immunological methods, 1989, 125(1-2): 279-285. [Content Brief]

[3]. Kim S H, et al. An improved protocol of biotinylated tyramine-based immunohistochemistry minimizing nonspecific background staining [J]. Journal of Histochemistry & Cytochemistry, 2003, 51(1): 129-132. [Content Brief]

[4]. Hunyady B, et al. Immunohistochemical signal amplification by catalyzed reporter deposition and its application in double immunostaining [J]. Journal of Histochemistry & Cytochemistry, 1996, 44(12): 1353-1362. [Content Brief]

[5]. Dráberová E, et al. Quantification of α-tubulin isotypes by sandwich ELISA with signal amplification through biotinyl-tyramide or immuno-PCR [J]. Journal of immunological methods, 2013, 395(1-2): 63-70. [Content Brief]

[6]. Kharel P, et al. Stress promotes RNA G-quadruplex folding in human cells [J]. Nature Communications, 2023, 14(1): 205. [Content Brief]

[7]. Pató A, et al. Hydrogen peroxide production by epidermal dual oxidase 1 regulates nociceptive sensory signals [J]. Redox Biology, 2023, 62: 102670. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.7513 mL	13.7563 mL	27.5126 mL	68.7815 mL
	5 mM	0.5503 mL	2.7513 mL	5.5025 mL	13.7563 mL
	10 mM	0.2751 mL	1.3756 mL	2.7513 mL	6.8781 mL
	15 mM	0.1834 mL	0.9171 mL	1.8342 mL	4.5854 mL
	20 mM	0.1376 mL	0.6878 mL	1.3756 mL	3.4391 mL
	25 mM	0.1101 mL	0.5503 mL	1.1005 mL	2.7513 mL
	30 mM	0.0917 mL	0.4585 mL	0.9171 mL	2.2927 mL
	40 mM	0.0688 mL	0.3439 mL	0.6878 mL	1.7195 mL
	50 mM	0.0550 mL	0.2751 mL	0.5503 mL	1.3756 mL
	60 mM	0.0459 mL	0.2293 mL	0.4585 mL	1.1464 mL
	80 mM	0.0344 mL	0.1720 mL	0.3439 mL	0.8598 mL
	100 mM	0.0275 mL	0.1376 mL	0.2751 mL	0.6878 mL