2. Others Cell Cycle/DNA Damage
  3. Fluorescent Dye DNA Stain
  4. Br-DAPI

Br-DAPI is a marker dye in DAPI series. DAPI is a fluorescent dye that binds strongly to DNA. It binds to the AT base pair of the double-stranded DNA minor groove, and one DAPI molecule can occupy three base pair positions. The fluorescence intensity of DAPI molecules bound to double-stranded DNA is increased by about 20 times, and it is commonly observed with fluorescence microscopy, and the amount of DNA can be determined based on the intensity of fluorescence. In addition, because DAPI can pass through intact cell membranes, it can be used to stain both live and fixed cells. Storage: Keep away from light.

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Br-DAPI Chemical Structure

Br-DAPI Chemical Structure

CAS No. : 2387906-44-5

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5 mg USD 200 In-stock
10 mg USD 320 In-stock
25 mg USD 650 In-stock
50 mg USD 1050 In-stock
100 mg USD 1680 In-stock
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Based on 3 publication(s) in Google Scholar

Other Forms of Br-DAPI:

Br-DAPI Related Antibodies

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  • Purity & Documentation

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Description

Br-DAPI is a marker dye in DAPI series. DAPI is a fluorescent dye that binds strongly to DNA. It binds to the AT base pair of the double-stranded DNA minor groove, and one DAPI molecule can occupy three base pair positions. The fluorescence intensity of DAPI molecules bound to double-stranded DNA is increased by about 20 times, and it is commonly observed with fluorescence microscopy, and the amount of DNA can be determined based on the intensity of fluorescence. In addition, because DAPI can pass through intact cell membranes, it can be used to stain both live and fixed cells[1]. Storage: Keep away from light.

Cellular Effect
Cell Line Type Value Description References
MCF7 CC50
> 64 μM
Compound: Br-DAPI
Photocytotoxicity against human MCF7 cells assessed as reduction in cell viability incubated for 1 hr under dark condition followed by washout and measured after 24 hrs by MTT assay
Photocytotoxicity against human MCF7 cells assessed as reduction in cell viability incubated for 1 hr under dark condition followed by washout and measured after 24 hrs by MTT assay
[PMID: 36480920]
MCF7 CC50
7.6 μM
Compound: Br-DAPI
Photocytotoxicity against human MCF7 cells assessed as reduction in cell viability incubated for 1 hr followed by washout and irradiation with 8 J/cm^2 UV irradiation for 5 mins and measured after 24 hrs by MTT assay
Photocytotoxicity against human MCF7 cells assessed as reduction in cell viability incubated for 1 hr followed by washout and irradiation with 8 J/cm^2 UV irradiation for 5 mins and measured after 24 hrs by MTT assay
[PMID: 36480920]
In Vitro

General Protocol
1. Preparation of DAPI working solution
1.1 Preparation of the stock solution
Dissolve 1 mg DAPI in 1 mL ddH2O to obtain 1 mg/mL of stock solution.
Note: It is recommended to store the stock solution at -20°C or -80°C away from light and avoid repetitive freeze-thaw cycles.
1.2 Preparation of DAPI working solution
Dilute the stock solution in serum-free cell culture medium or PBS to obtain 1-10 ug/mL of working solution.
Note: Please adjust the concentration of DAPI working solution according to the actual situation.
2. Cell staining
2.1 Suspension cells（6-well plate）
a. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.The cell density is 1×106/mL.
b. Add 1 mL of working solution, and then incubate at room temperature for 3-10 minutes.
c. Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.
d. Wash twice with PBS, 5 minutes each time.
e. Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry.
2.2 Adherent cells
a. Culture adherent cells on sterile coverslips.
b. Remove the coverslip from the medium and aspirate excess medium.
c. Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 3-10 minutes.
d. Wash twice with medium, 5 minutes each time.Observation by fluorescence microscopy or flow cytometry.

Storage
-20°C, 1 year. Protect from light

Precautions 1. Please adjust the concentration of DAPI working solution according to the actual situation.
2. This product is for R&D use only, not for drug, household, or other uses.
3. For your safety and health, please wear a lab coat and disposable gloves to operate.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Br-DAPI Related Antibodies
Molecular Weight

356.22

Formula

C16H14BrN5
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

N=C(C1=CC2=C(C=C1)C(Br)=C(C3=CC=C(C(N)=N)C=C3)N2)N
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (280.73 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.8073 mL 14.0363 mL 28.0725 mL
5 mM 0.5615 mL 2.8073 mL 5.6145 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration
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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

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C2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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g

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Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.8073 mL 14.0363 mL 28.0725 mL 70.1813 mL
5 mM 0.5615 mL 2.8073 mL 5.6145 mL 14.0363 mL
10 mM 0.2807 mL 1.4036 mL 2.8073 mL 7.0181 mL
15 mM 0.1872 mL 0.9358 mL 1.8715 mL 4.6788 mL
20 mM 0.1404 mL 0.7018 mL 1.4036 mL 3.5091 mL
25 mM 0.1123 mL 0.5615 mL 1.1229 mL 2.8073 mL
30 mM 0.0936 mL 0.4679 mL 0.9358 mL 2.3394 mL
40 mM 0.0702 mL 0.3509 mL 0.7018 mL 1.7545 mL
50 mM 0.0561 mL 0.2807 mL 0.5615 mL 1.4036 mL
60 mM 0.0468 mL 0.2339 mL 0.4679 mL 1.1697 mL
80 mM 0.0351 mL 0.1755 mL 0.3509 mL 0.8773 mL
100 mM 0.0281 mL 0.1404 mL 0.2807 mL 0.7018 mL
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Br-DAPI Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Br-DAPI2387906-44-5Fluorescent DyeDNA StainInhibitorinhibitorinhibit

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