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  3. Bromocresol purple

Bromocresol purple is a pH indicator, whose color changes from yellow to purple when the pH increases from 5.2 to 6.8. Bromocresol purple can be used for detecting yeast cells with plasma membrane damage and measuring serum albumin concentrations.

For research use only. We do not sell to patients.

Bromocresol purple Chemical Structure

Bromocresol purple Chemical Structure

CAS No. : 115-40-2

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Based on 1 publication(s) in Google Scholar

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Description

Bromocresol purple is a pH indicator, whose color changes from yellow to purple when the pH increases from 5.2 to 6.8. Bromocresol purple can be used for detecting yeast cells with plasma membrane damage and measuring serum albumin concentrations[1][2][3].

In Vitro

Arabidopsis thaliana (L.) seedlings are transferred to Murashige and Skoog (MS) or low phosphorus medium which contained 0.75% [weight/volume (w/v)] agar, 0.006% (w/v) Bromocresol purple, pH 5.8 for 8-24 h. There are no significant color changes around the roots of wild-type (WT) seedlings on the MS medium[1].
Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
1. The yeast cells were centrifuged, washed with distilled water and resuspended in 5mM-citrate-phosphate buffer, pH 4.6.
2. Aliquots containing 107 cells were suspended in the same buffer, supplemented with glucose to a final concentration of 220 mM and different volumes of killer toxin preparation were added so that the final volume was 1 mL.
3. Incubation with the killer was then carried out in Eppendorf vials at 25℃ on a shaker for 120 min.
4. 50 μL 10 mM Bromocresol purple in 5 mM-citrate-phosphate buffer, pH 4.6, was added to the samples and incubation continued for 60 min under the same conditions.
5. The samples were then centrifuged, thoroughly washed with 5 mM-citrate-phosphate buffer.
6. The fraction of stained cells was determined in a-Jenalumar fluorescence microscope using violet light for excitation. Cell fluorescence was observed using filter G 251, which cuts off wavelengths below 450 nm.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

540.22

Formula

C21H16Br2O5S

CAS No.
Appearance

Solid

Color

Light brown to brown

SMILES

O=S1(OC(C2=CC(C)=C(O)C(Br)=C2)(C3=CC(C)=C(O)C(Br)=C3)C4=CC=CC=C41)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Store at room temperature 3 years

In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

Ethanol : 12.5 mg/mL (23.14 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.8511 mL 9.2555 mL 18.5110 mL
5 mM 0.3702 mL 1.8511 mL 3.7022 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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This equation is commonly abbreviated as: C1V1 = C2V2

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Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.4%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
Ethanol 1 mM 1.8511 mL 9.2555 mL 18.5110 mL 46.2774 mL
5 mM 0.3702 mL 1.8511 mL 3.7022 mL 9.2555 mL
10 mM 0.1851 mL 0.9255 mL 1.8511 mL 4.6277 mL
15 mM 0.1234 mL 0.6170 mL 1.2341 mL 3.0852 mL
20 mM 0.0926 mL 0.4628 mL 0.9255 mL 2.3139 mL
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    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Bromocresol purple
Cat. No.:
HY-W087928
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