Bromothymol Blue sodium

Cat. No.: HY-D0012A: FAQs
Data Sheet Handling Instructions

Molarity Calculator

Bromothymol Blue sodium salt is a pH indicator. Storage: protect from light.

For research use only. We do not sell to patients.

Bromothymol Blue sodium Chemical Structure

CAS No. : 34722-90-2

Size		Stock
MOQ		Minimum order quantity
50 mg		Get quote
100 mg		Get quote
250 mg		Get quote
Other size		Get quote

Synthetic products have potential research and development risk.

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Other In-stock Forms of Bromothymol Blue sodium:

Bromothymol Blue

Other Forms of Bromothymol Blue sodium:

Bromothymol Blue In-stock

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

Biological Activity
Protocol
Purity & Documentation
References
Customer Review

Description	Bromothymol Blue sodium salt is a pH indicator. Storage: protect from light.
In Vitro	A first characterization and comparison is done by developing an easy and direct measurement method based on a pH indicator system using Bromothymol Blue (BTB) as the indicator and potassium phosphate as the buffer. A pH-shift assay is developed on the basis of Bromothymol Blue as the pH indicator and potassium phosphate as the buffer component^[1]. Three pH indicators are tested for the direct determination of 2- 2-keto-L-gulonic acid (2-KLG) production on a plate. The results show that Bromothymol Blue is superior to the other two indicators in terms of the obvious color change and a suitable pH range (blue to yellow at pH 6.5-7.5). Upon the addition of a Bromothymol Blue solution (0.1%, w/v) to an agar plate, zones surrounding colonies of K. vulgare 07 mutants change their color from blue to yellow because K. vulgare 07 mutants release 2-KLG on agar plates, thereby acidifying surrounding areas around colonies^[2]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Bromothymol Blue sodium Related Antibodies
Molecular Weight	646.36
Formula	C₂₇H₂₇Br₂NaO₅S
CAS No.	34722-90-2
Appearance	Solid
SMILES	O=S1(OC(C2=CC(C(C)C)=C(C(Br)=C2C)O)(C3=CC(C(C)C)=C(C(Br)=C3C)O[Na])C4=C1C=CC=C4)=O
Shipping	Room temperature in continental US; may vary elsewhere.
Storage	4°C, sealed storage, away from moisture and light *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Purity & Documentation	Data Sheet (283 KB) Handling Instructions (2659 KB)
References	[1]. Pick A, et al. Identification and characterization of two new 5-keto-4-deoxy-D-Glucarate Dehydratases/Decarboxylases. BMC Biotechnol. 2016 Nov 17;16(1):80. [Content Brief] [2]. Yang W, et al. A plate method for rapid screening of Ketogulonicigenium vulgare mutants for enhanced 2-keto-l-gulonic acid production. Braz J Microbiol. 2017 Jul - Sep;48(3):397-402. [Content Brief]

Kinase Assay
^[1]

For all three enzymes, an expression in the 96-deep well scale is performed. Therefore, electrocompetent E. coli ArcticExpress (DE3) cells are transformed with the corresponding plasmid. Single clones are picked using the CP7200 Colony Picker and transferred to 96-deep well plates filled with 1.2 mL autoinduction media by a MicroFlo Select dispenser. After incubation (36 h, 37°C at 1,000 rpm), further processing is done manually. First, 100 μL of cell culture is transferred into a 96-well plate (U-shaped bottom) and harvested by centrifugation (4,570 rpm, 10 min at RT) while the supernatant is discarded. The frozen pellets (1 h at -20°C) are thawed at room temperature for one hour to improve cell lysis. Lysis is continued by the addition of 30 μL lysis buffer (3 h, 1,000 rpm, 37°C) containing 2 mM KP_i, pH 7.0, 2 mM MgCl₂, 10 μg/mL DNaseI, 100 μg/mL lysozyme. Next, 120 μL buffer (2 mM KP_i, pH 7.0) is added followed by centrifugation (3,000 rpm, 15 min at RT). For the photometric measurement, 20 μL of the crude extract is transferred to a 96-well plate (F-shaped bottom) and the reaction is started by adding 180 μL master mix to give a final volume of 200 μL (2.5 mM KP_i, pH 7.0, 2 mM MgCl₂, 25 μg/mL Bromothymol Blue (BTB) and 5 mM keto-deoxy-D-glucarate). The measurements are carried out for 60 min at 2-min intervals. Depending on the enzyme, different time windows are used for the activity calculation^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Pick A, et al. Identification and characterization of two new 5-keto-4-deoxy-D-Glucarate Dehydratases/Decarboxylases. BMC Biotechnol. 2016 Nov 17;16(1):80. [Content Brief]

[2]. Yang W, et al. A plate method for rapid screening of Ketogulonicigenium vulgare mutants for enhanced 2-keto-l-gulonic acid production. Braz J Microbiol. 2017 Jul - Sep;48(3):397-402. [Content Brief]