BTO-1 

BTO-1 is a Polo-like kinase (Plk) inhibitor. BTO-1 is primarily used for phosphorylation and dephosphorylation applications^[1][2].

BTO-1 (50 μM; 4 hours; U20S cells) results in monopolar spindles in a comparable fraction of mitotic cells. BTO-1(20 μM; 1 hour; PTK cells) shows a dose-dependent reduction in phospho-Cdc25C. BTO-1(25 μM; 1 hour; PTK cells) shows about 20 % reduction in H3 phosphorylation compared to control cells. BTO-1 in HeLa cells results in a blockage of Rho and Rho-GEF recruitment, which is essential for the assembly of a functional contractile ring^[1][2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

264.22

C₉H₄N₄O₄S

40647-02-7

O=C(C1=[N+]([O-])C2=CC(C#N)=CC([N+]([O-])=O)=C2S1)N

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Peters, U., et al. Probing cell-division phenotype space and Polo-like kinase function using small molecules. Nat Chem Biol 2, 618–626 (2006).

  [2]. Brennan IM, et al. Polo-like kinase controls vertebrate spindle elongation and cytokinesis. PLoS One. 2007;2(5):e409. Published 2007 May 2.  [Content Brief]