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  3. Fluorescent Dye
  4. Calcium Green 1AM

Calcium Green 1AM is a cell-permeant fluorescent calcium indicator (Excitation 506 nm; Emission 531 nm). Calcium Green 1AM is converted to the fluorescent calcium indicator by intracellular esterases.

For research use only. We do not sell to patients.

Calcium Green 1AM Chemical Structure

Calcium Green 1AM Chemical Structure

CAS No. : 186501-28-0

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50 μg USD 74 In-stock

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Description

Calcium Green 1AM is a cell-permeant fluorescent calcium indicator (Excitation 506 nm; Emission 531 nm). Calcium Green 1AM is converted to the fluorescent calcium indicator by intracellular esterases[1].

Molecular Weight

1290.96

Formula

C59H53Cl2N3O26
CAS No.
Appearance

Solid

Color

Off-white to light yellow

Emission (Em)

512
Excitation (Ex)

488
SMILES

O=C1OC2(C3=CC(Cl)=C(C=C3OC4=CC(OC(C)=O)=C(C=C42)Cl)OC(C)=O)C5=CC=C(C(NC6=CC=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C(OCCOC7=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C=CC=C7)=C6)=O)C=C15
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 6.45 mg/mL (5.00 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.7746 mL 3.8731 mL 7.7462 mL
5 mM 0.1549 mL 0.7746 mL 1.5492 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

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Concentration (final)

C2

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V2

In Vivo Dissolution Calculator
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 95.00%

SDS (252 KB)

COA (272 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 0.7746 mL 3.8731 mL 7.7462 mL 19.3654 mL
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Calcium Green 1AM Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Calcium Green 1AM186501-28-0Fluorescent DyeInhibitorinhibitorinhibit

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