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  6. 7240-90-6

7240-90-6

X-GAL Chemical Structure

7240-90-6

Chemical Structure

  • X-GAL
  • Synonym(s): BCIG
  • CAS No: 7240-90-6
    Formula: C14H15BrClNO6
    Molecular Weight: 408.63
  • IUPAC Name: (2S,3R,4S,5R,6R)-2-((5-bromo-4-chloro-1H-indol-3-yl)oxy)-6-(hydroxymethyl)tetrahydro-2H-pyran-3,4,5-triol
  • InChIKey: OPIFSICVWOWJMJ-AEOCFKNESA-N
  • SMILES: O[C@H]([C@@H](O)[C@H]1O)[C@@H](O[C@@H]1CO)OC2=CNC3=C2C(Cl)=C(Br)C=C3

Biological Activity: X-GAL (BCIG) is a widely used chromogenic β-galactosidase substrate. X-GAL is a colorless compound until cleaved by β-galactosidase, at which point X-GAL turns to an insoluble and detectable blue compound, making X-GAL particularly useful in techniques such as blue-white screening for cloning in bacteria. X-GAL can also be used for detection of β-galactosidase activity[1][2].

Cat. No. Product Name Purity Description
HY-15934 X-GAL 99.88% X-GAL (BCIG) is a widely used chromogenic β-galactosidase substrate. X-GAL is a colorless compound until cleaved by β-galactosidase, at which point X-GAL turns to an insoluble and detectable blue compound, making X-GAL particularly useful in techniques such as blue-white screening for cloning in bacteria. X-GAL can also be used for detection of β-galactosidase activity.

References

[1]. Sanchez-Ramos J, et al. The X-gal caution in neural transplantation studies. Cell Transplant. 2000 Sep-Oct;9(5):657-67. [Content Brief]
[2]. Li S, et al. In-situ SERS readout strategy to improve the reliability of beta-galactosidase activity assay based on X-gal staining in shortening incubation times. Talanta. 2021 Nov 1;234:122689. [Content Brief]

Keywords:

X-GAL