CDK4/6-IN-3

Cat. No.: HY-126244: FAQs
Data Sheet Handling Instructions

Molarity Calculator

CDK4/6-IN-3 is a brain-penetrant CDK4/CDK6 inhibitor with K_is of <0.3 nM and 2.2 nM, respectively. CDK4/6-IN-3 inhibits CDK1 with a K_i of 110 nM. CDK4/6-IN-3 can be used for the treatment of glioblastoma.

For research use only. We do not sell to patients.

CDK4/6-IN-3 Chemical Structure

CAS No. : 2366237-37-6

Size		Stock
MOQ		Minimum order quantity
50 mg		Get quote
100 mg		Get quote
250 mg		Get quote
Other size		Get quote

Synthetic products have potential research and development risk.

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

View All CDK Isoform Specific Products:

View All Isoforms

CDK1 CDK2 CDK3 CDK4 CDK5 CDK6 CDK7 CDK8 CDK9 CDK11 CDK12 CDK13 CDK14 CDK16 CDK19 CDC CLK Pho85

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[1]

CDK4

0.3 nM (Ki)

CDK6

2.2 nM (Ki)

CDK1

110 nM (Ki)

Cellular Effect

Cell Line	Type	Value	Description	References
A 172	EC₅₀	0.27 μM Compound: 24	Antiproliferative activity against human A172 assessed as reduction in cell viability incubated for 5 days by CyQuantGR dye-based fluorescence assay Antiproliferative activity against human A172 assessed as reduction in cell viability incubated for 5 days by CyQuantGR dye-based fluorescence assay	[PMID: 31307887]
BT-549	EC₅₀	2.1 μM Compound: 24	Antiproliferative activity against human BT549 cells assessed as reduction in DNA replication incubated for 16 hours followed by addition of EdU and incubated for 8 hours by Alexa flour488 and HCS dye-based fluorescence imaging method Antiproliferative activity against human BT549 cells assessed as reduction in DNA replication incubated for 16 hours followed by addition of EdU and incubated for 8 hours by Alexa flour488 and HCS dye-based fluorescence imaging method	[PMID: 31307887]
BT-549	EC₅₀	4.4 μM Compound: 24	Cytotoxicity against human BT549 cells assessed as reduction in cell count after 24 hrs by high-throughput microscopy Cytotoxicity against human BT549 cells assessed as reduction in cell count after 24 hrs by high-throughput microscopy	[PMID: 31307887]
DBTRG-05MG	EC₅₀	0.18 μM Compound: 24	Antiproliferative activity against human DBTRG-05MG assessed as reduction in cell viability incubated for 5 days by CyQuantGR dye-based fluorescence assay Antiproliferative activity against human DBTRG-05MG assessed as reduction in cell viability incubated for 5 days by CyQuantGR dye-based fluorescence assay	[PMID: 31307887]
M059J	EC₅₀	2.46 μM Compound: 24	Antiproliferative activity against human M059J assessed as reduction in cell viability incubated for 5 days by CyQuantGR dye-based fluorescence assay Antiproliferative activity against human M059J assessed as reduction in cell viability incubated for 5 days by CyQuantGR dye-based fluorescence assay	[PMID: 31307887]
MCF7	EC₅₀	0.03 μM Compound: 24	Antiproliferative activity against human MCF7 cells assessed as reduction in DNA replication incubated for 16 hours followed by addition of EdU and incubated for 8 hours by Alexa flour488 and HCS dye-based fluorescence imaging method Antiproliferative activity against human MCF7 cells assessed as reduction in DNA replication incubated for 16 hours followed by addition of EdU and incubated for 8 hours by Alexa flour488 and HCS dye-based fluorescence imaging method	[PMID: 31307887]
MCF7	EC₅₀	4.1 μM Compound: 24	Cytotoxicity against human MCF7 cells assessed as reduction in cell count after 24 hrs by high-throughput microscopy Cytotoxicity against human MCF7 cells assessed as reduction in cell count after 24 hrs by high-throughput microscopy	[PMID: 31307887]
SF-539	EC₅₀	2.74 μM Compound: 24	Antiproliferative activity against human SF539 assessed as reduction in cell viability incubated for 5 days by CyQuantGR dye-based fluorescence assay Antiproliferative activity against human SF539 assessed as reduction in cell viability incubated for 5 days by CyQuantGR dye-based fluorescence assay	[PMID: 31307887]
T98G	EC₅₀	0.52 μM Compound: 24	Antiproliferative activity against human T98G assessed as reduction in cell viability incubated for 5 days by CyQuantGR dye-based fluorescence assay Antiproliferative activity against human T98G assessed as reduction in cell viability incubated for 5 days by CyQuantGR dye-based fluorescence assay	[PMID: 31307887]
U-87MG ATCC	EC₅₀	0.17 μM Compound: 24	Antiproliferative activity against human U87MG assessed as reduction in cell viability incubated for 5 days by CyQuantGR dye-based fluorescence assay Antiproliferative activity against human U87MG assessed as reduction in cell viability incubated for 5 days by CyQuantGR dye-based fluorescence assay	[PMID: 31307887]

In Vitro

CDK4/6-IN-3 (Compound 24) inhibits MCF7 and BT549 cell lines proliferation with EC₅₀s of 30 nM and 2.1 μM, respectively^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

462.57

Formula

C₂₅H₃₁FN₈

CAS No.

2366237-37-6

SMILES

FC1=CN=C(NC2=NC=C(N3CC4(CN(CC)C4)C3)C=C2)N=C1C5=C([C@H](C)CCCC6)N6N=C5

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Bronner SM, et al. Design of a brain-penetrant CDK4/6 inhibitor for glioblastoma. Bioorg Med Chem Lett. 2019 Aug 15;29(16):2294-2301. [Content Brief]

CDK4/6-IN-3 Related Classifications

Molarity Calculator
Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass		Concentration		Volume		Molecular Weight *
	=		×		×

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)	×	Volume _(start)	=	Concentration _(final)	×	Volume _(final)
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

CDK4/6-IN-32366237-37-6CDKCyclin dependent kinaseInhibitorinhibitorinhibit

Your Recently Viewed Products:

Product Name

Salutation

Applicant Name *

Email Address *

Phone Number *

Organization Name *

Department *

Requested quantity *

Country or Region *

Remarks