Cecropin P1, porcine acetate

Cat. No.: HY-P2317A: FAQs
Data Sheet Handling Instructions

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Cecropin P1, porcine acetate is an antibacterial peptide that can be isolated from the upper part of the small intestine of the pig. Cecropin P1, porcine acetate shows antibacterial activity against Gram-negative bacteria. Cecropin P1, porcine acetate shows antiviral activity and inhibits PRRSV infection.

For research use only. We do not sell to patients.

Cecropin P1, porcine acetate Chemical Structure

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Other Forms of Cecropin P1, porcine acetate:

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Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

Cecropin P1, porcine (0-480 μg/mL, 36-96 h) markedly inhibits CH-1a infection and replication in Marc-145 cells^[2].
Cecropin P1, porcine (0-480 μg/mL, 36 h) not only displays extracellular virucidal activity against PRRSV (porcine reproductive and respiratory syndrome virus), but also exerts a potent inhibitory effect when added either before, simultaneously with, or after viral inoculation^[2].
Cecropin P1, porcine (480 μg/mL, 0-72 h) blocks CH-1a-induced apoptosis during the late phase of infection^[2].
Cecropin P1, porcine (0-480 μg/mL, 0-4 h) inhibits viral particle release^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:	Marc-145 cells
Concentration:	160, 320, and 480 μg/mL
Incubation Time:	36, 48, 72, 96 h
Result:	Significantly inhibited viral infection in a dose-dependent manner at 36 h postinfection. Inhibited CH-1a infection in Marc-145 cells with a 50% effective concentration (EC₅₀) of 112 μg/mL. The 50% cytotoxic concentration (CC₅₀) of Cecropin P1 for Marc-145 cells was estimated to be 719 μg/mL.

Western Blot Analysis^[2]

Cell Line:	Marc-145 cells
Concentration:	160, 320, and 480 μg/mL
Incubation Time:	36 h
Result:	Significantly reduced the expression of the viral N protein when administered with either the pre-, co-, or posttreatment method.

In Vivo

Cecropin P1 (1 mg/kg, IP, once) prevents bacterial growth, endotoxemia, and mortality in rats with septic shock^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

3398.91

Formula

C₁₄₉H₂₅₇N₄₅O₄₅

Sequence Shortening

SWLSKTAKKLENSAKKRISEGIAIAIQGGPR

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent & Solubility

In Vitro:

H₂O

Peptide Solubility and Storage Guidelines:

1. Calculate the length of the peptide.

2. Calculate the overall charge of the entire peptide according to the following table:

	Contents	Assign value
Acidic amino acid	Asp (D), Glu (E), and the C-terminal -COOH.	-1
Basic amino acid	Arg (R), Lys (K), His (H), and the N-terminal -NH₂	+1
Neutral amino acid	Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q)	0

3. Recommended solution:

Overall charge of peptide	Details
Negative (<0)	1. Try to dissolve the peptide in water first. 2. If water fails, add NH₄OH (<50 μL). 3. If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (>0)	1. Try to dissolve the peptide in water first. 2. If water fails, try dissolving the peptide in a 10%-30% acetic acid solution. 3. If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0)	1. Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first. 2. For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Andersson M, et al. Ascaris nematodes from pig and human make three antibacterial peptides: isolation of cecropin P1 and two ASABF peptides. Cell Mol Life Sci. 2003 Mar;60(3):599-606. [Content Brief]

[2]. Guo C, et al. Cecropin P1 inhibits porcine reproductive and respiratory syndrome virus by blocking attachment. BMC Microbiol. 2014 Nov 18;14:273. [Content Brief]

[3]. Giacometti A, et al. Effect of mono-dose intraperitoneal cecropins in experimental septic shock. Crit Care Med. 2001 Sep;29(9):1666-9. [Content Brief]

[4]. Jiang R, et al. Expression of antimicrobial peptide Cecropin P1 in Saccharomyces cerevisiae and its antibacterial, antiviral activity in vitro. Electronic Journal of Biotechnology, 2020.