2. Apoptosis
  3. Apoptosis
  4. CLEFMA

CLEFMA is a curcuminoid with antitumor activity. CLEFMA inhibits tumor growth is associated with NF-κB-regulated anti-inflammatory and anti-metastatic effects.

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CLEFMA Chemical Structure

CLEFMA Chemical Structure

CAS No. : 1246964-32-8

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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10 mM * 1 mL in DMSO In-stock
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Based on 1 publication(s) in Google Scholar

CLEFMA Related Antibodies

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Description

CLEFMA is a curcuminoid with antitumor activity. CLEFMA inhibits tumor growth is associated with NF-κB-regulated anti-inflammatory and anti-metastatic effects[1][2].

Cellular Effect
Cell Line Type Value Description References
NCI-H441 IC50
1 μM
Compound: 29, CLEFMA
Antiproliferative activity against human H441 cells assessed as decrease of hexosaminidase activity after 24 hrs
Antiproliferative activity against human H441 cells assessed as decrease of hexosaminidase activity after 24 hrs
[PMID: 20638855]
In Vitro

CLEFMA (1-100 μM; 24-72 h) has anti-proliferative activity[1].
CLEFMA inhibits the viability of H441 and A549 cells, with IC50s of 6.4 and 8.9 μM, respectively[2].
CLEFMA (5-10 μM; 24 h) induces apoptosis in H441 and A549 cells[2].
CLEFMA (1 and 10 μM; 24 h) induces autophagic death in H441 cells[1].
CLEFMA (1-20 μM) reduces the DNA-binding activity of NF-κB in H441 cells in a dose-dependent manner[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

CLEFMA Related Antibodies

Cell Proliferation Assay[1]

Cell Line: PANC-1, MiaPaCa-2, PC-3 and H441 cells
Concentration: 1, 10, 25, 100 μM
Incubation Time: 24, 48, 72 h
Result: Inhibits cells proliferation in a dose-dependent manner.
In Vivo

CLEFMA (0.2-0.4 mg/kg; i.p. daily for 4 weeks) inhibits tumor growth and suppresses the uptake of FDG in tumor tissue in a xenograft mouse model[2].
CLEFMA (0.2-0.4 mg/kg; i.p. daily for 4 weeks) down-regulates the expression of anti-apoptotic markers cIAP1, Bcl-xL, Bcl-2 and survivin, and induces the cleavage of pro-apoptotic protein BID and the expression of pro-apoptotic BAX in tumor tissue[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male athymic nu/nu mice (4 weeks old) are injected H441 cells[2]
Dosage: 0.2, 0.4 mg/kg
Administration: I.p. daily for 4 weeks
Result: Inhibited tumor growth up to 96% tumor at dose of 0.4 mg/kg.
Molecular Weight

442.29

Formula

C23H17Cl2NO4
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(O)/C=C\C(N1C/C(C(/C(C1)=C/C2=CC=CC=C2Cl)=O)=C\C3=CC=CC=C3Cl)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 116.67 mg/mL (263.79 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.2610 mL 11.3048 mL 22.6096 mL
5 mM 0.4522 mL 2.2610 mL 4.5219 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.2610 mL 11.3048 mL 22.6096 mL 56.5240 mL
5 mM 0.4522 mL 2.2610 mL 4.5219 mL 11.3048 mL
10 mM 0.2261 mL 1.1305 mL 2.2610 mL 5.6524 mL
15 mM 0.1507 mL 0.7537 mL 1.5073 mL 3.7683 mL
20 mM 0.1130 mL 0.5652 mL 1.1305 mL 2.8262 mL
25 mM 0.0904 mL 0.4522 mL 0.9044 mL 2.2610 mL
30 mM 0.0754 mL 0.3768 mL 0.7537 mL 1.8841 mL
40 mM 0.0565 mL 0.2826 mL 0.5652 mL 1.4131 mL
50 mM 0.0452 mL 0.2261 mL 0.4522 mL 1.1305 mL
60 mM 0.0377 mL 0.1884 mL 0.3768 mL 0.9421 mL
80 mM 0.0283 mL 0.1413 mL 0.2826 mL 0.7065 mL
100 mM 0.0226 mL 0.1130 mL 0.2261 mL 0.5652 mL
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CLEFMA Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

CLEFMA1246964-32-8Apoptosiscurcuminoid antitumorNF-κB-regulatedanti-inflammatoryanti-metastaticcancerInhibitorinhibitorinhibit

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