2. Stem Cell/Wnt
  3. β-catenin Wnt
  4. CWP232228

CWP232228, a highly potent selective Wnt/β-catenin signaling inhibitor, antagonizes binding of β-catenin to T-cell factor (TCF) in the nucleus. CWP232228 suppresses tumor formation and metastasis without toxicity through the inhibition of the growth of breast and liver cancer stem cells (CSCs).

For research use only. We do not sell to patients.

CWP232228 Chemical Structure

CWP232228 Chemical Structure

CAS No. : 1144044-02-9

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1 mg USD 285 In-stock
5 mg USD 600 In-stock
10 mg USD 900 In-stock
25 mg USD 1710 In-stock
50 mg USD 2565 In-stock
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Based on 5 publication(s) in Google Scholar

CWP232228 Related Antibodies

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  • Biological Activity

  • Purity & Documentation

  • References

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Description

CWP232228, a highly potent selective Wnt/β-catenin signaling inhibitor, antagonizes binding of β-catenin to T-cell factor (TCF) in the nucleus. CWP232228 suppresses tumor formation and metastasis without toxicity through the inhibition of the growth of breast and liver cancer stem cells (CSCs)[1].

IC50 & Target

Wnt/β-catenin[1]
In Vitro

CWP232228 (0.01-100 μM; 48 hours) inhibits cell proliferation with IC50 values are 2 and 0.8 μM in mouse (4T1) and human (MDA-MB-435) breast cancer cell lines, respectively[1].
CWP232228 (0.01-10 μM; 48 hours) inhibits cell proliferation with IC50s of 2.566, 2.630, and 2.596 μM in Hep3B, Huh7 and HepG2 cells, respectively[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

CWP232228 Related Antibodies

Cell Proliferation Assay[1]

Cell Line: Mouse (4T1) and human (MDA-MB-435) breast cancer cell lines
Concentration: 0.01, 0.1, 1, 10, 100 μM
Incubation Time: 48 hours
Result: IC50s were 2 and 0.8 μM for 4T1 and MDA-MB-435 cell lines, respectively.

Cell Proliferation Assay[2]

Cell Line: Hepatocellular carcinoma cell lines HepG2, Huh7, and Hep3B
Concentration: 0.01, 0.1, 0.5, 1, 5, 10 μM
Incubation Time: 48 hours
Result: IC50s were 2.566, 2.630, and 2.596 μM for Hep3B, Huh7 and HepG2 cells, respectively.
In Vivo

CWP232228 (100 mg/kg, administered i.p.; daily; 21days for mice bearing 4T1 cell tumors; 60 days for mice bearing MDA-MB-435 cell tumors) results in a significant reduction in tumor volume[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: 7-week-old female Balb/c and NOD/SCID mice bearing 4T1 or MDA-MB-435 cell tumors[1]
Dosage: 100 mg/kg
Administration: Administered i.p.; daily; 21days for mice bearing 4T1 cell tumors, 60 days for mice bearing MDA-MB-435 cell tumors
Result: Treatment resulted in a significant reduction in tumor volume.
Molecular Weight

717.62

Formula

C33H34N7Na2O7P
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

C=CCN1CC(N([C@@H](CC2=CC=C(OP(O[Na])(O[Na])=O)C=C2)C(N(CC3=CC=CC4=CN(C)N=C43)C5)=O)[C@H]5N1C(NCC6=CC=CC=C6)=O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : 62.5 mg/mL (87.09 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.3935 mL 6.9675 mL 13.9350 mL
5 mM 0.2787 mL 1.3935 mL 2.7870 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 50 mg/mL (69.67 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 98.41%

COA (258 KB) LCMS (89 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 1.3935 mL 6.9675 mL 13.9350 mL 34.8374 mL
5 mM 0.2787 mL 1.3935 mL 2.7870 mL 6.9675 mL
10 mM 0.1393 mL 0.6967 mL 1.3935 mL 3.4837 mL
15 mM 0.0929 mL 0.4645 mL 0.9290 mL 2.3225 mL
20 mM 0.0697 mL 0.3484 mL 0.6967 mL 1.7419 mL
25 mM 0.0557 mL 0.2787 mL 0.5574 mL 1.3935 mL
30 mM 0.0464 mL 0.2322 mL 0.4645 mL 1.1612 mL
40 mM 0.0348 mL 0.1742 mL 0.3484 mL 0.8709 mL
50 mM 0.0279 mL 0.1393 mL 0.2787 mL 0.6967 mL
60 mM 0.0232 mL 0.1161 mL 0.2322 mL 0.5806 mL
80 mM 0.0174 mL 0.0871 mL 0.1742 mL 0.4355 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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CWP232228 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

CWP2322281144044-02-9CWP 232228CWP-232228β-cateninWntBeta cateninBreastliverCancerstemcellssignalingInhibitorinhibitorinhibit

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