Cyclothiazide

Cat. No.: HY-101165 Purity: 98.78%: FAQs
Data Sheet SDS COA Handling Instructions

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Cyclothiazide, a positive allosteric modulator of AMPA receptors, is used frequently to block the desensitization of both native and heterologously expressed AMPA receptors. Cyclothiazide is known to produce a fast inhibition of AMPA receptor desensitization and a much slower potentiation of the AMPA current.

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Cyclothiazide Chemical Structure

CAS No. : 2259-96-3

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 66	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 66	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 60	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 90	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 180	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 300	In-stock	Estimated Time of Arrival: December 31
100 mg		Get quote
200 mg		Get quote

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Based on 1 publication(s) in Google Scholar

Other Forms of Cyclothiazide:

Cyclothiazide (Standard) Get quote

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AMPA Receptor NMDA Receptor Kainate Receptor

Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
HEK293	EC₅₀	> 100 μM Compound: CTZ, Cyclothiazide	Activity at human recombinant GluA2 receptor flop isoform expressed in HEK293 cells assessed as effect on glutamate-induced calcium flux by Fluo-4/AM staining-based fluorescence assay Activity at human recombinant GluA2 receptor flop isoform expressed in HEK293 cells assessed as effect on glutamate-induced calcium flux by Fluo-4/AM staining-based fluorescence assay	[PMID: 20096591]
HEK293	EC₅₀	13.7 μM Compound: CTZ, Cyclothiazide	Activity at human recombinant GluA3 receptor flip isoform expressed in HEK293 cells assessed as effect on glutamate-induced calcium flux by Fluo-4/AM staining-based fluorescence assay Activity at human recombinant GluA3 receptor flip isoform expressed in HEK293 cells assessed as effect on glutamate-induced calcium flux by Fluo-4/AM staining-based fluorescence assay	[PMID: 20096591]
HEK293	EC₅₀	19.8 μM Compound: CTZ, Cyclothiazide	Activity at human recombinant GluA1 receptor flip isoform expressed in HEK293 cells assessed as effect on glutamate-induced calcium flux by Fluo-4/AM staining-based fluorescence assay Activity at human recombinant GluA1 receptor flip isoform expressed in HEK293 cells assessed as effect on glutamate-induced calcium flux by Fluo-4/AM staining-based fluorescence assay	[PMID: 20096591]
HEK293	EC₅₀	2.24 μM Compound: CTZ, Cyclothiazide	Activity at human recombinant GluA2 receptor flip isoform expressed in HEK293 cells assessed as effect on glutamate-induced calcium flux by Fluo-4/AM staining-based fluorescence assay Activity at human recombinant GluA2 receptor flip isoform expressed in HEK293 cells assessed as effect on glutamate-induced calcium flux by Fluo-4/AM staining-based fluorescence assay	[PMID: 20096591]
HEK293	EC₅₀	3.91 μM Compound: CTZ, Cyclothiazide	Activity at human recombinant GluA4 receptor flip isoform expressed in HEK293 cells assessed as effect on glutamate-induced calcium flux by Fluo-4/AM staining-based fluorescence assay Activity at human recombinant GluA4 receptor flip isoform expressed in HEK293 cells assessed as effect on glutamate-induced calcium flux by Fluo-4/AM staining-based fluorescence assay	[PMID: 20096591]
HEK293	EC₅₀	3800 nM Compound: 2, cyclothiazide	Activity against human GLUR4 flip expressed in HEK293 cells assessed as glutamate-stimulated calcium influx by FLIPR assay Activity against human GLUR4 flip expressed in HEK293 cells assessed as glutamate-stimulated calcium influx by FLIPR assay	[PMID: 16879964]
HEK293	EC₅₀	7.6 μM Compound: cyclothiazide	Agonist activity at human GluA2(Q)i expressed in HEK293 cells assessed as enhancement of glutamate-evoked desensitized current by whole cell patch clamp method Agonist activity at human GluA2(Q)i expressed in HEK293 cells assessed as enhancement of glutamate-evoked desensitized current by whole cell patch clamp method	[PMID: 26653877]
Oocyte	EC₅₀	10 μM Compound: CTZ, Cyclothiazide	Activity at recombinant GluA1 receptor flop isoform expressed in Xenopus oocytes co-expressing gamma2-TARP assessed as effect on 10 uM glutamate-induced current by voltage-clamp electrophysiological assay Activity at recombinant GluA1 receptor flop isoform expressed in Xenopus oocytes co-expressing gamma2-TARP assessed as effect on 10 uM glutamate-induced current by voltage-clamp electrophysiological assay	[PMID: 20096591]
Oocyte	EC₅₀	2 μM Compound: CTZ, Cyclothiazide	Activity at recombinant GluA1 receptor flip isoform expressed in Xenopus oocytes co-expressing gamma2-TARP assessed as effect on 10 uM glutamate-induced current by voltage-clamp electrophysiological assay Activity at recombinant GluA1 receptor flip isoform expressed in Xenopus oocytes co-expressing gamma2-TARP assessed as effect on 10 uM glutamate-induced current by voltage-clamp electrophysiological assay	[PMID: 20096591]
Oocyte	EC₅₀	7.6 μM Compound: Cyclothiazide	Agonist activity at rat flip iGluR2(Q) expressed in Xenopus laevis oocytes by whole cell patch-clamp method Agonist activity at rat flip iGluR2(Q) expressed in Xenopus laevis oocytes by whole cell patch-clamp method	[PMID: 18811139]

Molecular Weight

389.88

Formula

C₁₄H₁₆ClN₃O₄S₂

CAS No.

2259-96-3

Appearance

Solid

Color

White to off-white

SMILES

O=S(C1=C(Cl)C=C(C2=C1)NC(C3C(C4)C=CC4C3)NS2(=O)=O)(N)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (320.61 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.5649 mL	12.8245 mL	25.6489 mL
5 mM	0.5130 mL	2.5649 mL	5.1298 mL
10 mM	0.2565 mL	1.2824 mL	2.5649 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

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(per animal)

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(per animal)

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Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 98.78%

Select Batch:

Data Sheet (272 KB) SDS (393 KB)

COA (253 KB) HNMR (341 KB) LCMS (274 KB)

Handling Instructions (2659 KB)

References

[1]. Fucile S, et al. Effects of cyclothiazide on GluR1/AMPA receptors. Proc Natl Acad Sci U S A. 2006;103(8):2943-2947. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.5649 mL	12.8245 mL	25.6489 mL	64.1223 mL
	5 mM	0.5130 mL	2.5649 mL	5.1298 mL	12.8245 mL
	10 mM	0.2565 mL	1.2824 mL	2.5649 mL	6.4122 mL
	15 mM	0.1710 mL	0.8550 mL	1.7099 mL	4.2748 mL
	20 mM	0.1282 mL	0.6412 mL	1.2824 mL	3.2061 mL
	25 mM	0.1026 mL	0.5130 mL	1.0260 mL	2.5649 mL
	30 mM	0.0855 mL	0.4275 mL	0.8550 mL	2.1374 mL
	40 mM	0.0641 mL	0.3206 mL	0.6412 mL	1.6031 mL
	50 mM	0.0513 mL	0.2565 mL	0.5130 mL	1.2824 mL
	60 mM	0.0427 mL	0.2137 mL	0.4275 mL	1.0687 mL
	80 mM	0.0321 mL	0.1603 mL	0.3206 mL	0.8015 mL
	100 mM	0.0256 mL	0.1282 mL	0.2565 mL	0.6412 mL

Cyclothiazide Related Classifications

Neurological Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Cyclothiazide2259-96-3iGluRIonotropic glutamate receptorsAMPA receptor desensitizationpositive allosteric modulatorcurrent potentiationneuronesInhibitorinhibitorinhibit

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Cyclothiazide

Customer Review

Other Forms of Cyclothiazide:

Cyclothiazide Related Antibodies

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Complete Stock Solution Preparation Table

Cyclothiazide Related Classifications

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