2. Metabolic Enzyme/Protease
  3. Phosphatase
  4. D-3

D-3, a phosphorpeptide, is an efficient, simple, and specific iPSC-eliminating agent.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

D-3 Chemical Structure

D-3 Chemical Structure

CAS No. : 1967815-98-0

Size Price Stock Quantity
5 mg USD 180 In-stock
10 mg USD 290 In-stock
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100 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

D-3 Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

D-3, a phosphorpeptide, is an efficient, simple, and specific iPSC-eliminating agent[1].

In Vitro

D-3 prevents residual iPSC-induced teratoma formation in a mouse tumorigenicity assay[1].
D-3 induces obvious loss of viability in 201B7 cells, with half maximal inhibitory concentration value of 192.3 ± 57.4 μM[1].
D-3 induces an increase in the amount of activated elF2a, p38, and p44/42 MAPK, which are activated in response to cellular stress. D-3 also increases the number of Annexin-V and SYTOX-positive cells, indicating apoptotic and dead cells after D-3 treatment[1].
D-3 has little influence on various non-iPSCs, including hepatocytes and neurons[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

D-3 Related Antibodies

Cell Viability Assay[1]

Cell Line: Six human iPSCs lines and one human ESC line (khES1).
Concentration: 400 μM.
Incubation Time: 1-2 h.
Result: Sufficient to induce a viability loss (>99% in all iPSC lines and >95% in khES1).
Molecular Weight

870.88

Formula

C48H47N4O10P
CAS No.
Appearance

Solid

Color

White to off-white

Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture and light, under nitrogen

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)

Solvent & Solubility
In Vitro: 

H2O : ≥ 53.3 mg/mL (61.20 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.1483 mL 5.7413 mL 11.4826 mL
5 mM 0.2297 mL 1.1483 mL 2.2965 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 1.1483 mL 5.7413 mL 11.4826 mL 28.7066 mL
5 mM 0.2297 mL 1.1483 mL 2.2965 mL 5.7413 mL
10 mM 0.1148 mL 0.5741 mL 1.1483 mL 2.8707 mL
15 mM 0.0766 mL 0.3828 mL 0.7655 mL 1.9138 mL
20 mM 0.0574 mL 0.2871 mL 0.5741 mL 1.4353 mL
25 mM 0.0459 mL 0.2297 mL 0.4593 mL 1.1483 mL
30 mM 0.0383 mL 0.1914 mL 0.3828 mL 0.9569 mL
40 mM 0.0287 mL 0.1435 mL 0.2871 mL 0.7177 mL
50 mM 0.0230 mL 0.1148 mL 0.2297 mL 0.5741 mL
60 mM 0.0191 mL 0.0957 mL 0.1914 mL 0.4784 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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D-3 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

D-31967815-98-0D3D 3PhosphataseIPSC201B7elF2ap38p44/42MAPKSYTOXInhibitorinhibitorinhibit

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