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  4. D-Ile-Pro-Arg p-nitroanilide dihydrochloride

D-Ile-Pro-Arg p-nitroanilide dihydrochloride 

Cat. No.: HY-137837 Purity: 99.87%
Data Sheet
SDS
COA
Handling Instructions Technical Support

Solubility

In Vivo Dissolution Calculator

Help & FAQs

D-Ile-Pro-Arg p-nitroanilide dihydrochloride is a chromogenic peptide substrate of tissue plasminogen activator (tPA).

For research use only. We do not sell to patients.

D-Ile-Pro-Arg p-nitroanilide dihydrochloride Chemical Structure

D-Ile-Pro-Arg p-nitroanilide dihydrochloride Chemical Structure

CAS No. : 96323-41-0

Size Price Stock Quantity
5 mg USD 200 In-stock
10 mg USD 320 In-stock
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100 mg   Get quote  

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Description

D-Ile-Pro-Arg p-nitroanilide dihydrochloride is a chromogenic peptide substrate of tissue plasminogen activator (tPA)[1].

Molecular Weight

577.50

Formula

C23H38Cl2N8O5
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

[O-][N+](C(C=C1)=CC=C1NC([C@H](CCCNC(N)=N)NC([C@H]2N(CCC2)C([C@H](N)[C@H](C)CC)=O)=O)=O)=O.Cl.Cl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (173.16 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 100 mg/mL (173.16 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.7316 mL 8.6580 mL 17.3160 mL
5 mM 0.3463 mL 1.7316 mL 3.4632 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 1.7316 mL 8.6580 mL 17.3160 mL 43.2900 mL
5 mM 0.3463 mL 1.7316 mL 3.4632 mL 8.6580 mL
10 mM 0.1732 mL 0.8658 mL 1.7316 mL 4.3290 mL
15 mM 0.1154 mL 0.5772 mL 1.1544 mL 2.8860 mL
20 mM 0.0866 mL 0.4329 mL 0.8658 mL 2.1645 mL
25 mM 0.0693 mL 0.3463 mL 0.6926 mL 1.7316 mL
30 mM 0.0577 mL 0.2886 mL 0.5772 mL 1.4430 mL
40 mM 0.0433 mL 0.2165 mL 0.4329 mL 1.0823 mL
50 mM 0.0346 mL 0.1732 mL 0.3463 mL 0.8658 mL
60 mM 0.0289 mL 0.1443 mL 0.2886 mL 0.7215 mL
80 mM 0.0216 mL 0.1082 mL 0.2165 mL 0.5411 mL
100 mM 0.0173 mL 0.0866 mL 0.1732 mL 0.4329 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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D-Ile-Pro-Arg p-nitroanilide dihydrochloride Related Classifications

Help & FAQs
  • How should lyophilized recombinant proteins be reconstituted and stored?

    1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss. 2. Taking 10 μg as an example, first add 20 μL of reconstituted solution provided by MCE and use a pipette to gently resuspend the lyophilized protein until it is fully dissolved.. (For most proteins, the reconstitution solution we provide is sterile water. If a diluent other than water is required, it will be indicated in the product's Certificate of Analysis (COA).). 3. Add an additional 80 μL of buffer/culture medium containing carrier protein (either 0.1% BSA, 5% HSA, 10% FBS, or 5% trehalose), and then use a pipette to gently mix until uniform. The final concentration is should not be lower than 100 μg/mL. 4. Aliquot at least 20 μL per tube. 5. After aliquoting, store it frozen at a temperature ranging from -20ºC to -80ºC, and it can be preserved for 3 to 6 months.

  • How should solution-form recombinant proteins be stored?

    1. The product can be stored in its original form and diluted as needed upon use. 2. Alternatively, dilute with a buffer/culture medium containing a carrier protein (either 0.1% BSA, 5% HSA, 10% FBS, or 5% alginate), mix well by pipetting, and ensure that

  • Why is it necessary to add carrier proteins?

    Carrier proteins are commonly added to enhance the stability of recombinant proteins, preventing them from adhering to the walls of the container during freezing or thawing processes. Plastic tubes have a certain adsorptive capacity for proteins, which may lead to difficulty in separating the protein from the tube walls, resulting in a decrease in the actual concentration of the protein in the solution and thus affecting its activity. To minimize such losses, it is recommended to add a commonly used carrier protein solution prior to the long-term storage of recombinant protein products.

  • Carrier protein types and options?

    In cases where the carrier protein is not expected to influence the experimental outcomes, an appropriate carrier protein, such as 0.1% BSA (Bovine Serum Albumin), 5% HSA (Human Serum Albumin), 10% FBS (Fetal Bovine Serum), or 5% trehalose, can be incorpo

Keywords:

D-Ile-Pro-Arg p-nitroanilide96323-41-0Biochemical Assay ReagentstPA substrateInhibitorinhibitorinhibit

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D-Ile-Pro-Arg p-nitroanilide dihydrochloride
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