2. Cell Cycle/DNA Damage
  3. Polo-like Kinase (PLK)
  4. DAP-81

DAP-81 is an inhibitory agent targeting Polo-like kinases (Plks), a class of evolutionarily conserved serine/threonine kinases. DAP-81 dose-dependently increases the number of monopolar spindles in treated cells. High-resolution live-cell microscopy revealed that Plk activity is required for the assembly and maintenance of bipolar mitotic spindles. Plk inhibition destabilizes centromeric microtubules while stabilizing other spindle microtubules, leading to the formation of monopolar spindles. Further testing of compounds based on "privileged scaffolds" such as the DAP scaffold may lead to the discovery of new cell division probes and anti-microtubule agents.

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DAP-81 Chemical Structure

DAP-81 Chemical Structure

CAS No. : 794466-17-4

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DAP-81 Related Antibodies

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Description

DAP-81 is an inhibitory agent targeting Polo-like kinases (Plks), a class of evolutionarily conserved serine/threonine kinases. DAP-81 dose-dependently increases the number of monopolar spindles in treated cells. High-resolution live-cell microscopy revealed that Plk activity is required for the assembly and maintenance of bipolar mitotic spindles. Plk inhibition destabilizes centromeric microtubules while stabilizing other spindle microtubules, leading to the formation of monopolar spindles. Further testing of compounds based on "privileged scaffolds" such as the DAP scaffold may lead to the discovery of new cell division probes and anti-microtubule agents.

Cellular Effect
Cell Line Type Value Description References
BSC-1 EC50
5 μM
Compound: 1, DAP-81
Antimitotic activity against african green monkey BSC1 cells assessed as induction of monopolar spindles by immunofluorescence microscopy
Antimitotic activity against african green monkey BSC1 cells assessed as induction of monopolar spindles by immunofluorescence microscopy
[PMID: 17028580]
Molecular Weight

468.46

Formula

C25H20N6O4
CAS No.
SMILES

CC(NC1=CC=C(C=C1)NC2=NC=C(C(NC3=CC=CC=C3C(C4=CC=CC=C4)=O)=N2)[N+]([O-])=O)=O
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Room temperature in continental US; may vary elsewhere.
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Purity & Documentation
References
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DAP-81 Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

DAP-81794466-17-4DAP81DAP 81Polo-like Kinase (PLK)Inhibitorinhibitorinhibit

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