DMANI 

DMANI is an intramolecular charge transfer (ICT)-based mitochondria-targeted ratiometric fluorescent probe. DMANI can monitor mitochondrial peroxynitrite (ONOO⁻) in living cells^[1].

In PBS buffer (10 mM, pH = 7.4), the DMANI (5 μM) displays absorption bands at around 525 and 346 nm, but the addition of 30 μM ONOO⁻ significantly decreased the absorption, together with the appearance of a new peak at about 360 nm. In the emission spectra, a remarkably decreased fluorescence was observed at 692 nm and a new emission band at 444 nm appeared and increased rapidly in the case of DMANI with ONOO^−[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

482.40

C₂₅H₂₇IN₂

2978635-30-0

Solid

CN(C)C1=CC=C2C(C=CC(/C=C/C(C3(C)C)=[N+](C)C4=C3C=CC=C4)=C2)=C1.[I-]

Room temperature in continental US; may vary elsewhere.

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

• [1]. Yuting Du, et al. ICT-based fluorescent ratiometric probe for monitoring mitochondrial peroxynitrite in living cells. New J. Chem., 2021,45, 12915-12921.