1. Others Cell Cycle/DNA Damage
  2. Fluorescent Dye DNA Stain
  3. DRAQ5

DRAQ5 is a novel cell permeant and far red-fluorescing DNA probe. DRAQ5 excites at a wavelength of 647 nm, close to the Ex, and produces a fluorescence spectrum extending from 665 nm out to beyond 780 nm wavelengths. DRAQ5 fluorescence reflects cellular DNA content. DRAQ5 can be used in combination with FITC and RPE-labelled antibodies, without the need for fluorescence compensation.

For research use only. We do not sell to patients.

DRAQ5 Chemical Structure

DRAQ5 Chemical Structure

CAS No. : 254098-36-7

Size Price Stock Quantity
Solvent
20 μL USD 246 In-stock
Solvent
50 μL USD 490 In-stock
Solvent
200 μL USD 1260 In-stock
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Description

DRAQ5 is a novel cell permeant and far red-fluorescing DNA probe. DRAQ5 excites at a wavelength of 647 nm, close to the Ex, and produces a fluorescence spectrum extending from 665 nm out to beyond 780 nm wavelengths. DRAQ5 fluorescence reflects cellular DNA content. DRAQ5 can be used in combination with FITC and RPE-labelled antibodies, without the need for fluorescence compensation[1].

In Vitro

Mammalian cell in full culture medium staining methods[2]:
(1) Cell planking: Digestive separation of cells and resuspend in complete medium to a concentration of 2-4 × 105 cells/ml.
Note: Attached cell cultures (e.g., coverslip cultures or chambered wells) can be stained in a 1-2-ml staining volume overlayering a 4-cm2 surface area.
(2) Prepare staining solution: Add 4 µl of 5 mM DRAQ5 acidified stock per ml culture medium (20 µM final).
Note: Nuclear discrimination is achievable at 2.5 to 5 µM, and it is unlikely that concentrations >30 µM would be required.
(3) Fluorescence staining: Incubate 5 to 15 min at 37°C.
Note: Overstaining cannot occur.
(4)Wash (optional): Centrifuge cells 3 to 5 min at 800 × g, 37°C. Discard supernatant and resuspend in complete medium with 10 mM HEPES (HY-D0857) at 4 × 105 cells/ml.
(5) For flow cytometry: Use conventional pulse analysis for doublet discrimination and analyze parameters using appropriate software.
(6) For laser scanning microscopy: Collect fluorescence images using a 695 nm long-pass filter.
Fixed cells staining methods[2]:
(1) Fixed cells:Use 4% paraformaldehyde in PBS for 30 min with resuspension in an aqueous buffer (e.g., PBS).
(2) Fluorescence staining: similar concentrations of dye and similar incubation conditions can be used as for live cells.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

412.50

CAS No.
Appearance

Liquid (Density: 1.380±0.06 g/cm3)

Color

Blue to dark blue

SMILES

N/A

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Purity & Documentation

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DRAQ5
Cat. No.:
HY-D1742
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