CY5-SE (Synonyms: Cy5 NHS Ester; Sulfo-Cyanine5 Succinimidyl Ester)

Cat. No.: HY-D0819 Purity: ≥98.0%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Cy5-SE (Cy5 NHS Ester) is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides. This dye requires small amount of organic co-solvent (such as DMF or DMSO) to be used in labeling reaction. This reagent is ideal for very cost-efficient labeling of soluble proteins, as well as all kinds of peptides and oligonucleotides. This reagent also works well in organic solvents for small molecule labeling. Excitation (nm):649, Emission (nm): 670.

For research use only. We do not sell to patients.

CY5-SE Chemical Structure

CAS No. : 146368-14-1

Size	Price	Stock	Quantity
5 mg	USD 120	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 180	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 360	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 570	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 910	In-stock	Estimated Time of Arrival: December 31
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500 mg		Get quote

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Based on 15 publication(s) in Google Scholar

Other Forms of CY5-SE:

CY5-SE triethylamine salt Get quote

15 Publications Citing Use of MCE CY5-SE

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Biological Activity
Purity & Documentation
References
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Description

In Vitro

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Conjugation of SELP Analogues with Cy5-SE.
1. 1 mL of each 100 μg/mL SELP solution in 100 mM sodium bicarbonate buffer (pH 8.3) is mixed with 10 μL of 1.2 mg/mL Cy5 mono NHS-ester in DMSO and incubates for 2 hours on ice.
2. To quench the reaction, 50 μL of 1 M Tris-HCl (pH 8.0) is added to the reaction solution.
3. Reaction mixtures are loaded onto 1.5 mL Sephadex G-25 columns, and Cy5-conjugated SELPs are eluted by centrifugation for 3 minutes at 1050g^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

753.88

Formula

C₃₇H₄₃N₃O₁₀S₂

CAS No.

146368-14-1

Appearance

Solid

Color

Dark purple to black

Emission (Em)

670

Excitation (Ex)

633

SMILES

CC(/C(N1CCCCCC(ON2C(CCC2=O)=O)=O)=C\C=C\C=C\C3=[N+](CC)C(C=CC(S(=O)([O-])=O)=C4)=C4C3(C)C)(C)C5=C1C=CC(S(=O)(O)=O)=C5

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 25 mg/mL (33.16 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : ≥ 5.88 mg/mL (7.80 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.3265 mL	6.6324 mL	13.2647 mL
5 mM	0.2653 mL	1.3265 mL	2.6529 mL
10 mM	0.1326 mL	0.6632 mL	1.3265 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (3.32 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (3.32 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: Saline
Solubility: 25 mg/mL (33.16 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.10%

Select Batch:

Data Sheet (278 KB) SDS (393 KB)

COA (256 KB) HNMR (239 KB) LCMS (357 KB)

Handling Instructions (2659 KB)

Dyeing Example

References

[1]. Jeon HY, et al. Array-Based High-Throughput Analysis of Silk-Elastinlike Protein Polymer Degradation and C-Peptide Release by Proteases. Anal Chem. 2016;88(10):5398-5405. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	1.3265 mL	6.6324 mL	13.2647 mL	33.1618 mL
H₂O / DMSO	5 mM	0.2653 mL	1.3265 mL	2.6529 mL	6.6324 mL
DMSO	10 mM	0.1326 mL	0.6632 mL	1.3265 mL	3.3162 mL
	15 mM	0.0884 mL	0.4422 mL	0.8843 mL	2.2108 mL
	20 mM	0.0663 mL	0.3316 mL	0.6632 mL	1.6581 mL
	25 mM	0.0531 mL	0.2653 mL	0.5306 mL	1.3265 mL
	30 mM	0.0442 mL	0.2211 mL	0.4422 mL	1.1054 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

CY5-SE Related Classifications

Others

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

CY5-SE146368-14-1Cy5 NHS Ester Sulfo-Cyanine5 Succinimidyl EsterFluorescent Dyeesterreactivedyeamino-groupspeptidesproteinsoligonucleotidesInhibitorinhibitorinhibit

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CY5-SE (Synonyms: Cy5 NHS Ester; Sulfo-Cyanine5 Succinimidyl Ester)

Customer Review

Other Forms of CY5-SE:

CY5-SE Related Antibodies

15 Publications Citing Use of MCE CY5-SE

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

CY5-SE Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

CY5-SE Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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SAFETY DATA SHEET (SDS)

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