2. Metabolic Enzyme/Protease
  3. Ser/Thr Protease
  4. Endoproteinase Glu-C

Endoproteinase Glu-C  (Synonyms: V8 protease; Glu-C)

Cat. No.: HY-E70194
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Endoproteinase GluC (V8 protease) is a serine proteinase. Endoproteinase GluC is able to hydrolyze some serpins and all classes of mammalian immunoglobulins.

For research use only. We do not sell to patients.

Endoproteinase Glu-C Chemical Structure

Endoproteinase Glu-C Chemical Structure

CAS No. : 137010-42-5

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100 μg USD 600 In-stock

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Based on 1 publication(s) in Google Scholar

Other Forms of Endoproteinase Glu-C:

Endoproteinase Glu-C Related Antibodies

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Description

Endoproteinase GluC (V8 protease) is a serine proteinase. Endoproteinase GluC is able to hydrolyze some serpins and all classes of mammalian immunoglobulins[1].

In Vitro

This product can be used for: protein enzyme digestion sequencing analysis, peptide map analysis and peptide mass fingerprint analysis.
Reaction conditions
Reaction pH: active between 4.0-10.0
Optimum pH: 8.0-8.5
Inhibitors: Diisopropylfluorophosphate (DFP), α2-macroglobulin, and Nα-P-tosyl-L-lysine chloromethyl ketone (TLCK)
Molecular weight: 24.0±2.4 kDa (SDS-PAGE)
Protocol
1）Dissolution: 25-50 mM NH4HCO3 (pH 7.8) to dissolve the target protein. If the solubility is not good, the target protein can be denatured (adding urea, SDS, DTT or heating).
2）Enzyme digestion: The recommended ratio of V8 protease and target protein is 1/20-1/100 (W/W), enzyme digestion at 25℃ or 37℃ for 2-18 h.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Endoproteinase Glu-C Related Antibodies
CAS No.
Appearance

Oil

Color

Colorless to light yellow

SMILES

[Endoproteinase Glu-C]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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Endoproteinase Glu-C Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Endoproteinase Glu-C137010-42-5V8 protease Glu-CSer/Thr ProteaseSerine proteasesSerine endopeptidasesThreonine proteasesserine proteinaseserpinimmunoglobulinInhibitorinhibitorinhibit

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Endoproteinase Glu-C
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HY-E70194
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