2. Enzyme
  3. Tissue Dissociation Enzyme

Tissue Dissociation Enzyme

Tissue dissociating enzymes can remove tissue, including trypsin, collagenase series, dispase, thermolysin, etc.

Tissue dissociating enzymes are mainly used for:

• Dissociating tissues and digesting cells

Tissue Dissociation Enzyme (21):

Cat. No. Product Name CAS No.  
  • HY-E70005D
    Collagenase IV, Clostridium histolytica 9001-12-1  
    Collagenase, Type IV (EC 3.4.24.3) is a microbially derived matrix metalloproteinases (MMPs) and zinc peptidase. Collagenase, Type IV degrades type IV collagen and type VII collagen, the main components of the basement membrane, and can also decompose basement matrix and elastin.
  • HY-E70005B
    Collagenase, Type II 9001-12-1  
    Collagenase, Type II is a microbially derived matrix metalloproteinases (MMPs) and zinc peptidase. Collagenase, Type II breaksdown collagens1, 3, 5, 7, 8, 10, fibronectin, gelatin, aggrecann.
  • HY-E70005A
    Collagenase, Type I 9001-12-1  
    Collagenase, Type I is a microbially derived matrix metalloproteinases (MMPs) and zinc peptidase. Collagenase, Type I breaks down collagens 1, 3, 7, 8, 10, gelatin, proteoglycans, aggrecan.
  • HY-131577
    Neutral protease, Paenibacillus polymyxa 42613-33-2  
    Neutral protease, Paenibacillus polymyxa (Dispase II, Dispase) is a neutral protease and potent fibronectinase and type IV collagenase. Neutral protease, Paenibacillus polymyxa can be used to separate the intact epidermis from the dermis and intact epithelial sheets in culture from the substratum.
  • HY-P1748
    Thermolysin, Bacillus thermoproteolyticus rokko 9073-78-3  
    Thermolysin, Bacillus thermoproteolyticus rokko (EC 3.4.24.27) (TML) is a thermostable neutral metalloproteinase enzyme secreted by the Gram-positive bacteria Bacillus thermoproteolyticus. Thermolysin catalyzes the hydrolysis of peptide bonds containing hydrophobic residues.

    Optimal pH: 8.0. Considerably stable from pH 5 to 9.5.
    Optimal temperature : 70 °C
  • HY-E70005H
    Collagenase, Type VIII 9001-12-1  
    Collagenase, Type VIII is a mixed enzyme derived from Clostridium histolyticum that contains collagenase, a nonspecific protease, and a clostripain. Collagenase, Type VIII can hydrolyze Type VIII collagen and may be used to study the formation of atherosclerosis. Type VIII collagen is a regulator of endothelial cell differentiation and angiogenesis, a substrate for cell adhesion and migration such as smooth muscle cells, and may accumulate in atherosclerosis. After endotoxin activates the expression of Collagenase, Type VIII, it can reduce the production of Type VIII collagen and has the potential to inhibit atherosclerosis.
  • HY-E70005J
    Collagenase, Type VII 9001-12-1  
    Collagenase, Type VII (EC 3.4.24.3) is a collagenase that can be used to induce thalamic hemorrhage.
  • HY-E70005I
    Collagenase, Type VI 9001-12-1  
    Collagenase, Type VI (EC 3.4.24.3) is a collagenase that can degrade type VI collagen. Type VI collagen is a component of cell membranes in various tissues (such as skin, heart, blood vessels, cartilage, and synovial fluid). Excessive collagenase can cause extracellular matrix lesions. Collagenase is also a biomarker for tumor invasion and metastasis. Collagenase, Type VI can specifically act on the peptide bond between proline and glycine. This feature can be used to quickly and sensitively detect its concentration level in experiments using corresponding modified electrodes.
  • HY-O0004
    Collagenase I, from Clostridium histolyticum 9001-12-1  
    Collagenases are enzymes that break the peptide bonds in collagen. Collagenases are derived from the Clostridium histolyticum. Collagenases (Type I) are proteolytic enzymes that break peptide bonds in collagen and can be used for tissue digestion and dissociation.
  • HY-E70005C
    Collagenase, Type III 9001-12-1  
    Collagenase, Type III is a microbially derived matrix metalloproteinases (MMPs) and zinc peptidase. Collagenase, Type III breaksdown collagens1, 4, 9, 10, 14, fibronectin, MMP-9, gelatin, plasminogen, aggrecan,perlecan osteonectin
  • HY-E70130
    Snailase, Snail gastrointestinal 158736-45-9  
    Snailase, Snail gastrointestinal is an enzyme mixture composed of more than 20 enzymes, which is often used for enzymatic hydrolysis of purified flavonoid glycosides. Snailase can be obtained from the digestive tract and includes cellulase, sucrase, hemicellulase, pectinase, polygalacturonase, protease, etc.
  • HY-E70005E
    Collagenase, Type V 9001-12-1  
    Collagenase, Type V is a microbially derived matrix metalloproteinases (MMPs) and zinc peptidase. Collagenase, Type V breaksdown collagens1, 3, 5, 7, 8, 10, fibronectin, gelatin, aggrecann.
  • HY-129047B
    Recombinant Trypsin Solution 9002-07-7  
    Recombinant Trypsin Solution is an animal-free trypsin solution used to digest cells or tissues. Recombinant Trypsin Solution has high stability at room temperature and gentle digestion, and can be used to digest weakly adherent cells or stem cells under low serum or serum-free culture conditions. Compared with traditional trypsin digestion solution, Recombinant Trypsin Solution does not contain any animal-derived ingredients, is gentle and effective, and can replace the application of animal-derived trypsin in cell digestion.
  • HY-E70353
    Trypsin-Chymotrypsin 6:1  
    Trypsin-Chymotrypsin 6:1 is a biocatalyst and a key enzyme in new biocatalyst technology. Enzyme engineering focuses on enhancing enzyme reaction kinetics, substrate selectivity, and activity under harsh conditions such as low or high pH. By introducing stimulus responsiveness to these enzyme modifications, dynamic control of activity is also possible.
  • HY-B2220A
    Cellulase R-10 from Trichoderma Vride 9012-54-8  
    Cellulase R-10 from Trichoderma Vride is an enzyme that catalyze cellulolysis. Cellulase R-10 from Trichoderma Vride can be used for the isolation of protoplasts, for its ability to degrade cell walls.
  • HY-131577A
    Neutral protease I 42613-33-2  
    Neutral protease I (Dispase I) is a rapid, effective, gentle and neutral protease that can separate intact epidermis from the dermis. Neutral protease I can also separate intact epithelial sheets in culture from the substratum. Neutral protease I preserves the viability of the epithelial cells while cleaving the basement membrane zone region. Neutral protease I can also be used to prevent clumping in suspension cultures. Neutral protease I cleaves fibronectin and type IV collagen, but not laminin, type V collagen, serum albumin, or transferrin.
  • HY-E70351
    Trypsin-Chymotrypsin 1:1  
    Trypsin-Chymotrypsin 1:1 is a biocatalyst and a key enzyme in new biocatalyst technology. Enzyme engineering focuses on enhancing enzyme reaction kinetics, substrate selectivity, and activity under harsh conditions such as low or high pH. By introducing stimulus responsiveness to these enzyme modifications, dynamic control of activity is also possible.
  • HY-E70352
    Trypsin-Chymotrypsin 1:250  
    Trypsin-Chymotrypsin 1:250 is a biocatalyst and a key enzyme in new biocatalyst technology. Enzyme engineering focuses on enhancing enzyme reaction kinetics, substrate selectivity, and activity under harsh conditions such as low or high pH. By introducing stimulus responsiveness to these enzyme modifications, dynamic control of activity is also possible.
  • HY-E70369
    Collagenase H (Recombinant)  
    Collagenase H Recombinant is able to digest and break down collagen (especially collagen III) in the extracellular matrix (ECM). Collagenase H Recombinant is important in the isolation and function of rat pancreatic islets. Collagenase H Recombinant can be used in tissue engineering and cell culture.
  • HY-E70368
    Collagenase G (Recombinant)  
    Collagenase G Recombinant is involved in the islet isolation process, and digests specific collagens from the N-terminus, especially Col-V.