Flipper-TR probe 

Flipper-TR probe (Compound FliptR probe) is a fluorescent probe specifically designed to measure cell membrane tension. Flipper-TR probe reports changes in membrane tension through variations in its fluorescence lifetime. Flipper-TR probe is applicable to a wide range of organisms including bacteria, yeast, mammals, and plants^[1].

Usage Guidelines:
Storage: Flipper-TR should be stored at -20°C upon receipt.
Probe Preparation: Dissolve the probe in anhydrous DMSO to create a 1 mM stock solution, which should be stored at -20 °C or lower.
Staining Solution Preparation: Prior to use, dilute Flipper-TR to the desired concentration (typically starting at 1 µM) in cell culture medium.
Staining Protocol: After cells reach the desired density, replace the culture medium with the staining solution to cover all cells, then incubate at 37°C in a humidified atmosphere with 5 % CO₂ for 15 minutes before imaging.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

478.71

C₂₀H₁₄O₂S₆

Solid

Orange to reddish brown

CC1=CSC(C2=C3C(C)=C(C4=C(C)C(SC5=C6SC=C5C)=C6S4)S2)=C1S3(=O)=O

Room temperature in continental US; may vary elsewhere.

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

• [1]. Colom A, et al. A fluorescent membrane tension probe. Nat Chem. 2018 Nov;10(11):1118-1125.  [Content Brief]