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  3. Fluorescent Dye Reactive Oxygen Species TNF Receptor Interleukin Related SOD
  4. Fluorene

Fluorene is an orally active polycyclic aromatic hydrocarbon (PAH) and a precursor to other fluorene-based compounds. Fluorene and its derivatives serve as dye precursors for fluorene synthesis. In A549 cells, Fluorene induces oxidative stress and inflammatory responses by increasing ROS and SOD generation, exacerbating lipid peroxidation, modulating antioxidant enzyme activity, and upregulating the expression of pro-inflammatory factors TNF-α and IL-6. In vivo, Fluorene exhibits anxiolytic activity. Fluorene holds potential for research in inflammation and neurological disorders.

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Fluorene Chemical Structure

Fluorene Chemical Structure

CAS No. : 86-73-7

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Based on 1 publication(s) in Google Scholar

Other Forms of Fluorene:

Fluorene Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Fluorene

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TNFRSF1A/CD120a TNFRSF5/CD40 TNFRSF6/Fas/CD95 TNFRSF7/CD27 TNFRSF8/CD30 TNFRSF9/4-1BB/CD137 TNFRSF16/NGF Receptor/CD271 TNFRSF18/GITR/CD357

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Description

Fluorene is an orally active polycyclic aromatic hydrocarbon (PAH) and a precursor to other fluorene-based compounds. Fluorene and its derivatives serve as dye precursors for fluorene synthesis. In A549 cells, Fluorene induces oxidative stress and inflammatory responses by increasing ROS and SOD generation, exacerbating lipid peroxidation, modulating antioxidant enzyme activity, and upregulating the expression of pro-inflammatory factors TNF-α and IL-6. In vivo, Fluorene exhibits anxiolytic activity. Fluorene holds potential for research in inflammation and neurological disorders[1][2][3].

Cellular Effect
Cell Line Type Value Description References
MDA-MB-231 IC50
> 50 μM
Compound: Fluorene
Cytotoxicity against triple-negative human MDA-MB-231 cells assessed as reduction in cell viability measured after 48 hrs by CCK8 assay
Cytotoxicity against triple-negative human MDA-MB-231 cells assessed as reduction in cell viability measured after 48 hrs by CCK8 assay
[PMID: 32726747]
In Vitro

Fluorene (200-800 μM, 24-48 hours) induces oxidative stress and inflammation in A549 cells, characterized by increased ROS generation, enhanced lipid peroxidation, altered antioxidant enzyme activity, and upregulated TNF-α and IL-6 expression[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Fluorene Related Antibodies

Western Blot Analysis[1]

Cell Line: A549 human lung epithelial cells (lung injury)
Concentration: 200, 400, 600, 800 μM
Incubation Time: 24 hours, 48 hours
Result: Upregulated TNF-α and IL-6 mRNA and protein expression levels. Additionally, SP-A expression increased in Fluorene-treated groups (400 and 600 μM) but decreased in the PAH mixture-treated group (600 μM).

Cell Viability Assay[1]

Cell Line: A549 human lung epithelial cells (lung injury)
Concentration: 200, 400, 600, 800 μM
Incubation Time: 24 hours, 48 hours
Result: Induced ROS generation in A549 cells, increased MDA content, and enhanced SOD and CAT activity.
In Vivo

Fluorene (100 mg/kg, p.o. or i.p., once daily for 60 days) decreases weight gain and increases relative liver weight in Wistar rats, with the i.p. route resulting in higher blood and brain levels of Fluorene and its metabolites[3].
Fluorene (1, 10 mg/kg/day, p.o. or i.p., once daily for 60 days) reduces anxiety levels in Wistar rats without affecting locomotor activity or learning ability[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Fluorene oral or intraperitoneal (i.p.) administration-induced body weight and organ changes in Wistar rats[1]
Dosage: 1, 10, 100 mg/kg
Administration: Oral gavage (p.o.) or intraperitoneal injection (i.p.), once daily for 60 days
Result: Exhibited significantly reduced weight gain after 28 days and increased relative liver weight (The 100 mg/kg group). Additionally, higher concentrations of Fluorene and its hydroxylated metabolites were detected in the blood and brain of i.p.-treated rats compared to p.o.-treated ones.
Reduced anxiety levels in rats (1, 10 mg/kg), while the high dose (100 mg/kg) showed no significant changes. No effects on locomotor activity or learning ability were observed.
Molecular Weight

166.22

Formula

C13H10
CAS No.
Appearance

Solid

Color

Off-white to light brown

SMILES

C1(C=CC=C2)=C2CC3=C1C=CC=C3
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

RT, protect from light

In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 62.5 mg/mL (376.00 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 6.0160 mL 30.0801 mL 60.1601 mL
5 mM 1.2032 mL 6.0160 mL 12.0320 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

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Purity & Documentation

Purity: 99.35%

SDS (623 KB)

COA (254 KB) HNMR (129 KB) RP-HPLC (331 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 6.0160 mL 30.0801 mL 60.1601 mL 150.4004 mL
5 mM 1.2032 mL 6.0160 mL 12.0320 mL 30.0801 mL
10 mM 0.6016 mL 3.0080 mL 6.0160 mL 15.0400 mL
15 mM 0.4011 mL 2.0053 mL 4.0107 mL 10.0267 mL
20 mM 0.3008 mL 1.5040 mL 3.0080 mL 7.5200 mL
25 mM 0.2406 mL 1.2032 mL 2.4064 mL 6.0160 mL
30 mM 0.2005 mL 1.0027 mL 2.0053 mL 5.0133 mL
40 mM 0.1504 mL 0.7520 mL 1.5040 mL 3.7600 mL
50 mM 0.1203 mL 0.6016 mL 1.2032 mL 3.0080 mL
60 mM 0.1003 mL 0.5013 mL 1.0027 mL 2.5067 mL
80 mM 0.0752 mL 0.3760 mL 0.7520 mL 1.8800 mL
100 mM 0.0602 mL 0.3008 mL 0.6016 mL 1.5040 mL
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Fluorene Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Fluorene86-73-7Fluorescent DyeReactive Oxygen SpeciesTNF ReceptorInterleukin RelatedSODTumor Necrosis Factor ReceptorTNFRILSuperoxide DismutasePAHdyeprecursorpolycyclic aromatic hydrocarbonWistar ratsA549 human lung epithelial cells (lung injury)ROSTNF-αoxidative stressInhibitorinhibitorinhibit

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