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  3. Fluorescent Dye
  4. Fluorescein-CM2

Fluorescein-CM2 is a fluorogenic molecule that can be used to rapidly screen esterase cut sites for protein-protein interaction-dependent (PPI-dependent) esterase activity in E. coli.

For research use only. We do not sell to patients.

Fluorescein-CM2 Chemical Structure

Fluorescein-CM2 Chemical Structure

CAS No. : 1394821-44-3

Size Price Stock Quantity
1 mg USD 714 In-stock
5 mg USD 2857 In-stock
10 mg USD 4000 In-stock
25 mg USD 6000 In-stock
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100 mg   Get quote  

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Based on 1 publication(s) in Google Scholar

Fluorescein-CM2 Related Antibodies

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Description

Fluorescein-CM2 is a fluorogenic molecule that can be used to rapidly screen esterase cut sites for protein-protein interaction-dependent (PPI-dependent) esterase activity in E. coli[1].

Molecular Weight

556.56

Formula

C32H28O9
CAS No.
Appearance

Solid

Color

Off-white to light yellow

SMILES

O=C1OC2(C3=C1C=CC=C3)C4=CC=C(C=C4OC5=CC(OCOC(C6(CC6)C)=O)=CC=C52)OCOC(C7(CC7)C)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (179.68 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.7968 mL 8.9838 mL 17.9675 mL
5 mM 0.3594 mL 1.7968 mL 3.5935 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

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g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.7968 mL 8.9838 mL 17.9675 mL 44.9188 mL
5 mM 0.3594 mL 1.7968 mL 3.5935 mL 8.9838 mL
10 mM 0.1797 mL 0.8984 mL 1.7968 mL 4.4919 mL
15 mM 0.1198 mL 0.5989 mL 1.1978 mL 2.9946 mL
20 mM 0.0898 mL 0.4492 mL 0.8984 mL 2.2459 mL
25 mM 0.0719 mL 0.3594 mL 0.7187 mL 1.7968 mL
30 mM 0.0599 mL 0.2995 mL 0.5989 mL 1.4973 mL
40 mM 0.0449 mL 0.2246 mL 0.4492 mL 1.1230 mL
50 mM 0.0359 mL 0.1797 mL 0.3594 mL 0.8984 mL
60 mM 0.0299 mL 0.1497 mL 0.2995 mL 0.7486 mL
80 mM 0.0225 mL 0.1123 mL 0.2246 mL 0.5615 mL
100 mM 0.0180 mL 0.0898 mL 0.1797 mL 0.4492 mL
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Fluorescein-CM2 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Fluorescein-CM21394821-44-3Fluorescent DyePPI-dependentE. coliesteraseInhibitorinhibitorinhibit

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