Fluorescein tyramide 

Fluorescein tyramide is a green fluorescent reagent (λ_abs: 494 nm; λ_em: 517 nm). Fluorescein tyramide is widely used for tyramide signal amplification (TSA) with a low-abundance in IHC, ICC, in situ hybridization (FISH) and flow cytometry (FCM) applications^[1][2].

HRP catalyzes localized deposition of multiple tyramide molecules (catalyzed reporter deposition, CARD), binding the fluorescein tyramide to adjacent tyrosines to enhance fluorescent signal.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

495.49

C₂₉H₂₁NO₇

210236-90-1

Solid

White to yellow

O=C1OC2(C3=C(OC4=C2C=CC(O)=C4)C=C(O)C=C3)C5=C1C=CC=C5.O=C(C)NCCC6=CC=C(O)C=C6

Room temperature in continental US; may vary elsewhere.

• [1]. A H Hopman, et al. Rapid synthesis of biotin-, digoxigenin-, trinitrophenyl-, and fluorochrome-labeled tyramides and their application for In situ hybridization using CARD amplification. J Histochem Cytochem. 1998 Jun;46(6):771-7.  [Content Brief]

  [2]. R P van Gijlswijk, et al. Fluorochrome-labeled tyramides: use in immunocytochemistry and fluorescence in situ hybridization. J Histochem Cytochem. 1997 Mar;45(3):375-82.  [Content Brief]