Flutax-2 (5/6-mixture) 

Flutax-2 (5/6-mixture) is an active fluorescent derivative of paclitaxel. Flutax-2 (5/6-mixture) binds to a polymerized α,β tubulin dimer. Excitation/emission wavelength: 496/524 nm. Paclitaxel, a diterpenoid secondary metabolite produced by Taxus species, can be used for the research of a variety of cancers^[1].

Preparation of Flutax-2 working solution
1.1 Preparation of the stock solution
Dissolve 10 mg of Flutax-2 in 1 mL of DMSO.
Note: It is recommended to store the stock solution at -20℃or -80℃away from light and avoid repetitive freeze-thaw cycles.
1.2 Preparation of Flutax-2 working solution
Dilute the stock solution in sacetate buffer to obtain 100 nM-1 μM of Flutax-2 working solution.
Note: Please adjust the concentration of Flutax-2 working solution according to the actual situation.
Cell staining
2.1 For suspension cells: Centrifuge at 1000 g at 4℃for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
For adherent cells: Discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4℃for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
2.2 Add 1 mL of Flutax-2 working solution, and then incubate at room temperature for 10-30 minutes.
2.3 Centrifuge at 400 g at 4℃for 3-4 minutes and then discard the supernatant.
2.4 Wash twice with PBS, 5 minutes each time.
2.5 Resuspend cells with serum-free cell culture medium or PBS, and then detect by fluorescence microscope or flow cytometer.
Storage
-20℃, 1 year
Protect from light

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

1319.27

C₁₄₂H₁₂₈F₄N₄O₄₂

Solid

Light yellow to yellow

520

495

O=C1OC2(C3=CC=C(C(N[C@H](C(O[C@H]([C@](C([C@H](OC(C)=O)C4=C5C)=O)(C)[C@@]6([C@H](OC(C7=CC=CC=C7)=O)[C@@](C[C@@H]5OC([C@@H]([C@@H](NC(C8=CC=CC=C8)=O)C9=CC=CC=C9)O)=O)(O)C4(C)C)[H])C[C@@]%10([H])OC[C@]%106OC(C)=O)=O)C)=O)C=C31)C%11=CC(F)=C(O)C=C%11OC%12=CC(O)=C(F)C=C2%12.O=C%13OC%14(C%15=CC(C(N[C@H](C(O[C@H]([C@](C([C@H](OC(C)=O)C%16=C%17C)=O)(C)[C@@]%18([C@H](OC(C%19=CC=CC=C%19)=O)[C@@](C[C@@H]%17OC([C@@H]([C@@H](NC(C%20=CC=CC=C%20)=O)C%21=CC=CC=C%21)O)=O)(O)C%16(C)C)[H])C[C@@]%22([H])OC[C@]%22%18OC(C)=O)=O)C)=O)=CC=C%15%13)C%23=CC(F)=C(O)C=C%23OC%24=CC(O)=C(F)C=C%14%24

Room temperature in continental US; may vary elsewhere.

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

• [1]. P B Vieira, et al. Analysis of microtubule cytoskeleton distribution using a fluorescent taxoid in two trichomonadid protozoa: Trichomonas gallinae and Tritrichomonas foetus. Exp Parasitol. 2008 May;119(1):186-91.  [Content Brief]

  [2]. P B Vieira, et al. Analysis of microtubule cytoskeleton distribution using a fluorescent taxoid in two trichomonadid protozoa: Trichomonas gallinae and Tritrichomonas foetus. Exp Parasitol. 2008 May;119(1):186-91.  [Content Brief]