Fructosyl-lysine dihydrochloride  (Synonyms: Fructoselysine dihydrochloride)

Fructosyl-lysine (Fructoselysine) dihydrochloride is an amadori glycation product from the reaction of glucose and lysine by the Maillard reaction. Fructosyl-lysine dihydrochloride is the precursor to glucosepane, a lysine–arginine protein cross-link that can be an indicator in diabetes detection^[1].

IC50: precursor to glucosepane^[2]

Fructosyl-lysine dihydrochloride (5 mM; 0.5 hours) catalyzes the ATP-dependent conversion of [¹⁴C]fructoselysine to anionic products suggesting the existence of a fructoselysine-kinase activity in E .coli extracts^[2].
Fructosyl-lysine dihydrochloride (100 μM; 1 hour) contains a carbohydrate moiety and appears to be phosphorylated, it can be converted to glucose 6-phosphate in bacterial extracts in E .coli extracts^[2].
Fructosyl-lysine dihydrochloride (25 mM; 25 hours) lets E. coli growth at a rate of about one-third of that observed with glucose as a carbon source. Lysine itself does not support growth in the absence of other carbon source and does not affect the growth observed with glucose^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Fructosyl-lysine dihydrochloride and AGE residues is increased markedly in glomeruli, retina, sciatic nerve, and plasma protein in diabetic rats^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

381.25

C₁₂H₂₆Cl₂N₂O₇

96192-35-7

Solid

Brown to black

N[C@H](C(O)=O)CCCCNCC([C@@H](O)[C@H](O)[C@H](O)CO)=O.[H]Cl.[H]Cl

Room temperature in continental US; may vary elsewhere.

4°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

• [1]. Rabbani N, et al. Hidden complexities in the measurement of fructosyl-lysine and advanced glycation end products for risk prediction of vascular complications of diabetes. Diabetes. 2015 Jan;64(1):9-11.  [Content Brief]

  [2]. Karachalias N, et al. Accumulation of fructosyl-lysine and advanced glycation end products in the kidney, retina and peripheral nerve of streptozotocin-induced diabetic rats. Biochem Soc Trans. 2003 Dec;31(Pt 6):1423-5.  [Content Brief]