Fura-2 pentasodium 

Fura-2 pentasodium is a scaled fluorescent dye that can be used for intracellular calcium imaging with the K_d value of 140 nM. Fura-2 pentasodium has an emission wavelength of 510 nm and excitation wavelengths of 340 nm or 380 nm and the ratio of 340/380 fluorescence intensity is proportional to the intracellular Ca²⁺ level^[1][2].

Fura-2 (10 μM, 2 min) pentasodium can be used to detect changes in the concentration of calcium ions in neutrophils of heparinized peripheral blood^[1].
Fura-2 (10 μM, 5 min) pentasodium can be used as a calcium probe to assess the distribution of calcium ions within heart mitochondria without affecting the activity of it in rat^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

751.45

C₂₉H₂₂N₃Na₅O₁₄

O=C(O[Na])C(O1)=CN=C1C(O2)=CC(C=C3OCCOC4=C(N(CC(O[Na])=O)CC(O[Na])=O)C=CC(C)=C4)=C2C=C3N(CC(O[Na])=O)CC(O[Na])=O

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Omar Rafael Alemán, et al. The Antibody Receptor Fc Gamma Receptor IIIb Induces Calcium Entry via Transient Receptor Potential Melastatin 2 in Human Neutrophils. Front Immunol. 2021 May 13;12:657393. doi: 10.3389/fimmu.2021.657393.  [Content Brief]

  [2]. James G. McCormack, et al. Studies on mitochondrial Ca2+-transport and matrix Ca2+ using fura-2-loaded rat heart mitochondria. Biochim Biophys Acta. 1989 Mar 23;973(3):420-7.  [Content Brief]