Nos3 - nitric oxide synthase 3 Gene

Rattus norvegicus

Also known as eNos

Gene ID: 24600 | Gene type: protein coding

About Nos3

Primary_assembly 4: 10,793,834-10,814,166 reverse strand.mRatBN7.2:CM026977.1

This gene has 2 transcripts (splice variants), 114 orthologues, 5 paralogues and is associated with 177 phenotypes. Biased expression in Heart (RPKM 114.2), Spleen (RPKM 51.1) and 8 other tissues.

Summary

Enables several functions, including HSP90 protein binding activity; beta-catenin binding activity; and Calmodulin binding activity. Involved in several processes, including cellular response to magnesium ion; cellular response to mechanical stimulus; and cellular response to organonitrogen compound. Located in several cellular components, including caveola; nucleolus; and sarcolemma. Used to study artery disease (multiple); irritable bowel syndrome; ischemia (multiple); liver disease (multiple); and retinal disease (multiple). Biomarker of several diseases, including artery disease (multiple); kidney failure (multiple); liver disease (multiple); lung disease (multiple); and overnutrition (multiple). Human ortholog(s) of this gene implicated in several diseases, including Alzheimer's disease (multiple); artery disease (multiple); eye disease (multiple); reproductive organ Cancer (multiple); and vasculitis (multiple). Orthologous to human NOS3 (nitric oxide synthase 3). [provided by Alliance of Genome Resources, Apr 2022]

Nos3 Products(1)

mRNA	Protein	Name
NM_021838.2	NP_068610.1	nitric oxide synthase, endothelial

Gene Ontology

Molecular Function
Biological Process
Cellular Component

Molecular Function GO Annotation	Evidence	Reference	Source
enables Hsp90 protein binding	IDA IDA: Inferred from direct assay	15475520	RGD
enables actin binding	IDA IDA: Inferred from direct assay	15858215	RGD
enables beta-catenin binding	IPI IPI: Inferred from physical interaction	15858215	RGD
enables cadherin binding	IPI IPI: Inferred from physical interaction	15858215	RGD
enables calmodulin binding	IDA IDA: Inferred from direct assay	7545663	RGD
enables nitric-oxide synthase activity	IDA IDA: Inferred from direct assay	12388111	RGD
enables nitric-oxide synthase activity	IMP IMP: Inferred from mutant phenotype	12388111	RGD
enables nitric-oxide synthase binding	IPI IPI: Inferred from physical interaction	15858215	RGD
enables protein binding	IPI IPI: Inferred from physical interaction	17123860	RGD
enables scaffold protein binding	IPI IPI: Inferred from physical interaction	12566108	RGD

Biological Process GO Annotation	Evidence	Reference	Source
involved in bone development	IEP IEP: Inferred from expression pattern	10352094	RGD
involved in cellular response to cAMP	IEP IEP: Inferred from expression pattern	8621775	RGD
involved in cellular response to growth factor stimulus	IEP IEP: Inferred from expression pattern	18023142	RGD
involved in cellular response to heat	IEP IEP: Inferred from expression pattern	15475520	RGD
involved in cellular response to high density lipoprotein particle stimulus	IEP IEP: Inferred from expression pattern	17918268	RGD
involved in cellular response to insulin stimulus	IEP IEP: Inferred from expression pattern	29055722	RGD
involved in cellular response to low-density lipoprotein particle stimulus	IEP IEP: Inferred from expression pattern	29161690	RGD
involved in cellular response to magnesium ion	IEP IEP: Inferred from expression pattern	15919495	RGD
involved in cellular response to mechanical stimulus	IMP IMP: Inferred from mutant phenotype	19673940	RGD
involved in cellular response to organic cyclic compound	IEP IEP: Inferred from expression pattern	18006476	RGD
involved in cellular response to rotenone	IEP IEP: Inferred from expression pattern	28145852	RGD
involved in cellular response to transforming growth factor beta stimulus	IMP IMP: Inferred from mutant phenotype	21792926	RGD
involved in cellular response to tumor necrosis factor	IEP IEP: Inferred from expression pattern	22566503	RGD
involved in female pregnancy	IEP IEP: Inferred from expression pattern	15642797	RGD
involved in negative regulation of smooth muscle cell proliferation	IMP IMP: Inferred from mutant phenotype	12388111	RGD
involved in nitric oxide biosynthetic process	IEP IEP: Inferred from expression pattern	16344378	RGD
involved in nitric oxide biosynthetic process	IMP IMP: Inferred from mutant phenotype	12388111	RGD
involved in nitric oxide metabolic process	IEP IEP: Inferred from expression pattern	31489946	RGD
involved in positive regulation of apoptotic process	IMP IMP: Inferred from mutant phenotype	21792926	RGD
involved in response to L-arginine	IEP IEP: Inferred from expression pattern	28966130	RGD
involved in response to activity	IEP IEP: Inferred from expression pattern	19793134	RGD
involved in response to amino acid	IEP IEP: Inferred from expression pattern	17535904	RGD
involved in response to axon injury	IEP IEP: Inferred from expression pattern	15735325	RGD
involved in response to carbohydrate	IEP IEP: Inferred from expression pattern	29298510	RGD
involved in response to corticosterone	IEP IEP: Inferred from expression pattern	11304519	RGD
involved in response to cytokine	IEP IEP: Inferred from expression pattern	17591083	RGD
involved in response to erythropoietin	IEP IEP: Inferred from expression pattern	29122552	RGD
involved in response to estradiol	IDA IDA: Inferred from direct assay	20233295	RGD
involved in response to estradiol	IEP IEP: Inferred from expression pattern	18004295	RGD
involved in response to ethanol	IEP IEP: Inferred from expression pattern	18284813	RGD
involved in response to fructose	IEP IEP: Inferred from expression pattern	12154112	RGD
involved in response to hyperoxia	IEP IEP: Inferred from expression pattern	17937862	RGD
involved in response to hypoxia	IEP IEP: Inferred from expression pattern	17947499	RGD
involved in response to ketone	IEP IEP: Inferred from expression pattern	19717731	RGD
involved in response to lead ion	IEP IEP: Inferred from expression pattern	17403586	RGD
involved in response to lipopolysaccharide	IEP IEP: Inferred from expression pattern	18031380	RGD
involved in response to magnetism	IEP IEP: Inferred from expression pattern	28979692	RGD
involved in response to mechanical stimulus	IEP IEP: Inferred from expression pattern	19329460	RGD
involved in response to mercury ion	IEP IEP: Inferred from expression pattern	28980197	RGD
involved in response to metal ion	IEP IEP: Inferred from expression pattern	19446049	RGD
involved in response to nicotine	IEP IEP: Inferred from expression pattern	29236702	RGD
involved in response to nutrient levels	IEP IEP: Inferred from expression pattern	29055722	RGD
involved in response to organic cyclic compound	IEP IEP: Inferred from expression pattern	17850874	RGD
involved in response to peptide	IEP IEP: Inferred from expression pattern	15525466	RGD
involved in response to peptide hormone	IEP IEP: Inferred from expression pattern	17895290	RGD
involved in response to salt	IEP IEP: Inferred from expression pattern	21746791	RGD
involved in response to ultrasound	IEP IEP: Inferred from expression pattern	28979692	RGD
involved in response to xenobiotic stimulus	IEP IEP: Inferred from expression pattern	18390819	RGD
involved in retina development in camera-type eye	IEP IEP: Inferred from expression pattern	16639026	RGD

Cellular Component GO Annotation	Evidence	Reference	Source
located in apical part of cell	IDA IDA: Inferred from direct assay	17123860	RGD
located in caveola	IDA IDA: Inferred from direct assay	29217667	RGD
located in cytoplasm	IDA IDA: Inferred from direct assay	11943139	RGD
located in nucleus	IDA IDA: Inferred from direct assay	11943139	RGD
located in sarcolemma	IDA IDA: Inferred from direct assay	9111020	RGD

EXP: Inferred from Experiment IDA: Inferred from direct assay IPI: Inferred from physical interaction IMP: Inferred from mutant phenotype IGI: Inferred from genetic interaction IEP: Inferred from expression pattern

Nos3 Protein Structure

NOS_oxygenase_euk

Flavodoxin_1

FNR_like

0
200
400
600
800
1000
1202 a.a.

Protein Preferred Names

Protein Names

nitric oxide synthase, endothelial

EC-NOS	NOS type III
NOSIII	cNOS
constitutive NOS	endothelial NOS
endothelial nitric oxide synthase 3	nitric oxide synthase 3, endothelial cell

Related Agents

Clinical Phase

Bioactive Molecules (1)

Cat. No.	Product Name	Effect	Purity	Phase
HY-18732A	L-NMMA acetate	Inhibitor	≥99.0%	Phase 4

Pre-clinical Phase

Bioactive Molecules (4)

Cat. No.	Product Name	Effect	Purity	Rare Diseases
HY-RS09441	Nos3 Mouse Pre-designed siRNA Set A	Pre-designed Sets	/	Unkown
HY-N8761	Kihadanin A	Inhibitor	≥97.0%	Unkown
HY-RS09440	NOS3 Human Pre-designed siRNA Set A	Pre-designed Sets	/	Unkown
HY-RS09442	Nos3 Rat Pre-designed siRNA Set A	Pre-designed Sets	/	Unkown

Orthologs Information

Species	Symbol	Source	ID
Homo sapiens	Nos3	NCBI	NCBI:4846

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