hCAIX/XII-IN-5

Cat. No.: HY-150695: FAQs
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hCAIX/XII-IN-5 (Coumarin 9a) a carbonic anhydrase (CA) inhibitor, and exhibits excellent hCA IX/XII selectivity (K_i=93.9 and 85.7 nM, respectively) over hCA I and hCA II. hCAIX/XII-IN-5 shows anti-proliferative activities to cancer cells. hCAIX/XII-IN-5 can delay cell cycle and induce apoptosis.

For research use only. We do not sell to patients.

hCAIX/XII-IN-5 Chemical Structure

CAS No. : 2421127-99-1

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CA CA I CA Ⅱ CA Ⅸ CA XII

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

hCA XII

85.7 nM (Ki)

hCA IX

93.9 nM (Ki)

In Vitro

hCAIX/XII-IN-5 (2.36-32.17 μM) treatment shows both anti-proliferative activities to MCF-7 and PANC-1 cells under the normoxic and hypoxic condition^[1].
hCAIX/XII-IN-5 (2.69 μM; 24 h) treatment shows accumulation of MCF-7 cells in the Sub-G₁ phase and cell arrest in G₂-M phase^[1].
hCAIX/XII-IN-5 (2.69 μM; 24 h) treatment increases the percent of annexin V-FITC-positive apoptotic cells, including both the early and late apoptotic cells (UR+LR) ^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:	MCF-7 and PANC-1 cells
Concentration:	2.36-32.17 μM
Incubation Time:
Result:	Exerted anti-proliferative activities to MCF-7 and PANC-1 cells (IC₅₀=2.69 μM and 32.17 μM, respectively) under the normoxic condition, and moderate activitits against both MCF-7 and PANC-1 cells (IC₅₀=16.36 μM and 11.78 μM, respectively) under the hypoxic condition.

Cell Cycle Analysis^[1]

Cell Line:	MCF-7 cells
Concentration:	2.69 μM
Incubation Time:	24 hours
Result:	Resulted in a significant increase in the percentage of cells at Sub-G₁ by 14-folds with significant increase in cell percentage in the G₁-M phase by 2.3-folds compared to the control.

Apoptosis Analysis^[1]

Cell Line:	MCF-7 cells
Concentration:	2.69 μM
Incubation Time:	24 hours
Result:	Resulted in a significant increase in the percent of annexin V-FITC-positive apoptotic cells, including both the early (from 0.61% to 6.77%) and late apoptotic (from 0.27% to 14.84%) cells (UR+LR), about 11- and 55-fold increase, respectively, as compared with the control.

Molecular Weight

291.30

Formula

C₁₈H₁₃NO₃

CAS No.

2421127-99-1

SMILES

O=C1C(C(/C=C/NC2=CC=CC=C2)=O)=CC3=C(O1)C=CC=C3

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Hany S Ibrahim Insights into the effect of elaborating coumarin-based aryl enaminones with sulfonamide or carboxylic acid functionality on carbonic anhydrase inhibitory potency and selectivity. Bioorg Chem. 2022 Sep;126:105888. [Content Brief]

hCAIX/XII-IN-5 Related Classifications

Molarity Calculator
Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass		Concentration		Volume		Molecular Weight *
	=		×		×

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)	×	Volume _(start)	=	Concentration _(final)	×	Volume _(final)
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

hCAIX/XII-IN-52421127-99-1Carbonic AnhydraseApoptosisCarbonate dehydrataseanti-proliferative activityMCF-7PANC-1delay cell cycleapoptosisInhibitorinhibitorinhibit

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