HDAC6-IN-44

Cat. No.: HY-162630: FAQs
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HDAC6-IN-44 (compound H10) is a selective HDAC6 inhibitor with an IC₅₀ value of 8.97 nM. HDAC6-IN-44 can inhibit the idiopathic pulmonary fibrosis (IPF) phenotype and exhibits antifibrotic activity. Additionally, HDAC6-IN-44 reduces fibrogenesis in a bleomycin-induced pulmonary fibrosis mouse model and demonstrates good metabolic stability. HDAC6-IN-44 holds promise for research in the field of idiopathic pulmonary fibrosis.

For research use only. We do not sell to patients.

HDAC6-IN-44 Chemical Structure

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•Related Small Molecules:

•Related Proteins:

View All HDAC Isoform Specific Products:

View All Isoforms

HDAC HDAC1 HDAC2 HDAC3 HDAC4 HDAC5 HDAC6 HDAC7 HDAC8 HDAC9 HDAC10 HDAC11 HD1 HD2

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[1]

HDAC6

8.97 nM (IC₅₀)

HDAC1

878 nM (IC₅₀)

HDAC2

3144 nM (IC₅₀)

HDAC4

847 nM (IC₅₀)

HDAC7

894 nM (IC₅₀)

HDAC10

1319 nM (IC₅₀)

HDAC11

>5000 nM (IC₅₀)

In Vitro

HDAC6-IN-44 (compound H10) (2.5-10 μM, 1-300 μM, 48 h) inhibits TGF-β-induced proliferation of HELF cells, cell proliferation and their HDAC6 activity in a dose-dependent manner without causing significant cytotoxic effects^[1].
HDAC6-IN-44 interferes with the TGF-β1-related fibrosis process by acting on HDAC6-related signaling pathways^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	HELF cells
Concentration:	2.5-10 μM (cell proliferation test), 1-300 μM (survival toxicity test)
Incubation Time:	48 h
Result:	Inhibited TGF-β-induced HELF cell proliferation without significantly inhibiting cell survival at a maximum concentration of approximately 10 μM.

Western Blot Analysis^[1]

Cell Line:	HELF cells
Concentration:	2.5-10 μM
Incubation Time:	48 h
Result:	Exhibited dose-dependent increase in acetylated α-tubulin expression and has no effect on histone H3 acetylation, while attenuating the elevated expression of all marker proteins induced by TGF-β1.

In Vivo

HDAC6-IN-44 (compound H10) (0.1 mL/10g, i.p., daily for 3 weeks) significantly inhibits the increase in HDAC6 activity and collagen deposition in lung tissue in a mouse model of pulmonary fibrosis^[1].
HDAC6-IN-44 (0.1 mL/10g, i.p., daily for 3 weeks) can inhibit the progression of pulmonary fibrosis in a dose-dependent manner in a mouse model of pulmonary fibrosis^[1].
Cell Viability Assay^[1]

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Bleomycin-induced pulmonary fibrosis mouse model^[1]
Dosage:	0.1 mL/10g
Administration:	Intraperitoneal injection (i.p.) , daily for 3 weeks
Result:	Caused the blue collagen fiber deposition to be spot-like, with the surrounding alveolar cavity slightly collapsed and the alveolar wall thickened, but the collagen fiber deposition was significantly less than that in the model group. Additionally, HDAC6-IN-44 could dose-dependently inhibit the expression of α-SMA and p-Smad2/3 in BLM-induced pulmonary fibrosis in mice.

Molecular Weight

351.40

Formula

C₂₀H₂₁N₃O₃

SMILES

O=C(NO)C1=CC=C(CN2C([C@@](CCC3)([H])N3CC4=CC=CC=C42)=O)C=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Li Y, et al. Discovery of novel pyrrolo[2,1-c][1,4]benzodiazepine-3,11-dione (PBD) derivatives as selective HDAC6 inhibitors for the efficient treatment of idiopathic pulmonary fibrosis (IPF) in vitro and in vivo. Eur J Med Chem. 2024 Jun 17;275:116608. [Content Brief]

HDAC6-IN-44 Related Classifications

Inflammation/Immunology

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

HDAC6-IN-44HDACHistone deacetylasesHELF cellsBleomycin-induced pulmonary fibrosis mouse modelIdiopathic pulmonary fibrosisInhibitorinhibitorinhibit

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