Hedgehog IN-6

Cat. No.: HY-162372: FAQs
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Hedgehog IN-6 (Compound Q29) is a Hedgehog (Hh) inhibitor that can be used in cancer research. Hedgehog IN-6 inhibits the hedgehog (Hh) pathway by binding to the cysteine-rich domain (CRD) of Smoothened (Smo) and blocking its cholesterization.

For research use only. We do not sell to patients.

Hedgehog IN-6 Chemical Structure

CAS No. : 2916444-33-0

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Description

In Vitro

Q29 (1-10 μM; 24 h) dose-dependently inhibits GLI1 protein expression activated by Shh-N^[1].
Q29 (1-10 μM; 24 h + 12 h) inhibits Hh signaling-dependent cell proliferation in a dose-dependent manner^[1].
Q29 inhibits Gli-luciferase activity with an IC₅₀ of 1.33 μM in the presence of different concentrations of Shh-N^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:	NIH 3T3 cells
Concentration:	1, 3, 10 μM
Incubation Time:	24 h
Result:	Dose dependently decreased the protein and mRNA levels of endogenous Gli1.

Cell Proliferation Assay^[1]

Cell Line:	cGNP cells; medulloblastoma cells
Concentration:	1, 3, 10 μM
Incubation Time:	24 h+12h
Result:	Dose dependently inhibited the growth of Shh-N-stimulated cGNP cells and medulloblastoma cells isolated from Ptch1+/P53/ mice.

In Vivo

Q29 (90 mg/kg; oral gavage; twice daily; 13 days) effectively inhibits tumor growth in Ptch1+/P53/ mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Ptch1+/P53/ mice^[1]
Dosage:	90 mg/kg
Administration:	p.o.; twice daily; 13 days
Result:	Effectively inhibited tumor growth and exhibited synergistic inhibition with vismodegib (15 mg/kg; HY-10440)

Molecular Weight

389.61

Formula

C₂₅H₄₃NO₂

CAS No.

2916444-33-0

SMILES

CC1=C(CCC(NCCCC)=O)[C@]2([H])CC[C@@]3([H])C(C)(C)[C@@H](O)CC[C@]3(C)[C@@]2([H])CC1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Liu YB, et al. A sterol analog inhibits hedgehog pathway by blocking cholesterylation of smoothened. Cell Chem Biol. 2024 Feb 28:S2451-9456(24)00076-X. [Content Brief]

Hedgehog IN-6 Related Classifications

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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