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  4. Heparinase II

Heparinase II is a biochemical reagent that can be used as a biological material or organic compound for life science related research.

For research use only. We do not sell to patients.

Heparinase II Chemical Structure

Heparinase II Chemical Structure

CAS No. : 149371-12-0

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10 U USD 270 In-stock
25 U USD 545 In-stock
50 U USD 873 In-stock

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Based on 1 publication(s) in Google Scholar

Heparinase II Related Antibodies

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Description

Heparinase II is a biochemical reagent that can be used as a biological material or organic compound for life science related research.

In Vitro

Product Information
Calcium ions inhibit the activity of heparinase II. Heparinase II cleaves sulfated polysaccharide chains containing 1-4 linkages between hexosamine and uronic acid residues (including iduronic acid and glucuronic acid residues) by an elimination mechanism. The reaction generates oligosaccharide products (mainly disaccharides) containing unsaturated uronic acids, which can be detected by UV spectroscopy at 232 nm. The enzyme also cleaves the antithrombin III binding pentasaccharide domain in the heparin molecule.

Dissolution
Dissolved in water at 1 mg/mL, fresh preparation is recommended. Or dissolve according to the specific experimental reference.

Reaction Conditions
1. pH stability range 5.0-9.0, optimum pH 7.0-7.5
2. Temperature stability range 20-37℃, optimum temperature 30℃
Note: When enzymatically cleaving the substrate, the incubation time and enzyme concentration need to be determined based on actual experience.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Heparinase II Related Antibodies
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

[Heparinase II]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation

SDS (251 KB)

COA (242 KB)

Handling Instructions (2659 KB)
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Heparinase II Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Heparinase II149371-12-0Biochemical Assay Reagentsbiochemical reagentbiological materialorganic compoundlife scienceInhibitorinhibitorinhibit

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Heparinase II
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HY-P2955
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