2. Others Immunology/Inflammation NF-κB Metabolic Enzyme/Protease
  3. Fluorescent Dye Reactive Oxygen Species
  4. HKPerox-2

HKPerox-2 is a highly sensitive green fluorescent probe for the specific detection of H2O2 in living cells with a maximum excitation wavelength and emission wavelength of 520 nm and 543 nm, respectively.

For research use only. We do not sell to patients.

HKPerox-2 Chemical Structure

HKPerox-2 Chemical Structure

CAS No. : 2245230-79-7

Size Price Stock Quantity
1 mg USD 375 In-stock
5 mg USD 990 In-stock
10 mg USD 1628 In-stock
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Customer Review

Based on 3 publication(s) in Google Scholar

HKPerox-2 Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

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Description

HKPerox-2 is a highly sensitive green fluorescent probe for the specific detection of H2O2 in living cells with a maximum excitation wavelength and emission wavelength of 520 nm and 543 nm, respectively[2].

In Vitro

1. Preparation of HKPerox-2 working solution
1.1 Preparation of the stock solution
Dissolve 1 mg HKPerox-2 in 172 μL DMF to obtain 10 mM of stock solution.
Note: It is recommended to store the stock solution at -20℃ -80℃ away from light and avoid repetitive freeze-thaw cycles.
1.2 Preparation of HKPerox-2 working solution
Dilute the stock solution in serum-free cell culture medium or PBS to obtain 1-10 μM of working solution.
Note: Please adjust the concentration of HKPerox-2 working solution according to the actual situation.
2. Cell staining
2.1 Suspension cells (6-well plate)
a. Centrifuge at 1000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.The cell density is 1×106/mL
b. Add 1 mL of working solution, and then incubate at room temperature for 5-30 minutes.
c. Centrifuge at 400 g at 4℃ for 3-4 minutes and then discard the supernatant.
d. Wash twice with PBS, 5 minutes each time.
e. Resuspend cells with serum-free cell culture medium or PBS.Observation by fluorescence microscopy or flow cytometry.
2.2 Adherent cells
a. Culture adherent cells on sterile coverslips.
b. Remove the coverslip from the medium and aspirate excess medium.
c. Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 5-30 minutes.
d. Wash twice with medium, 5 minutes each time.Observation by fluorescence microscopy or flow cytometry.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

HKPerox-2 Related Antibodies
Molecular Weight

581.53

Formula

C32H23NO10
CAS No.
Appearance

Solid

Color

Yellow to orange

Emission (Em)

543
Excitation (Ex)

520
SMILES

O=CC1=C(O)C=C(COC(N(C)C2=CC=C3C(OC4=CC(OC)=CC=C4C53OC(C6=CC=C(C(O)=O)C=C65)=O)=C2)=O)C=C1.O=CC7=C(O)C=C(COC(N(C)C8=CC=C9C(OC%10=CC(OC)=CC=C%10C%119OC(C%12=CC(C(O)=O)=CC=C%12%11)=O)=C8)=O)C=C7.[1:1]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : ≥ 100 mg/mL (171.96 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

DMF : 50 mg/mL (85.98 mM; ultrasonic and warming and heat to 60°C)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.7196 mL 8.5980 mL 17.1960 mL
5 mM 0.3439 mL 1.7196 mL 3.4392 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.79%

SDS (252 KB)

COA (264 KB) HNMR (332 KB) RP-HPLC (266 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMF / DMSO 1 mM 1.7196 mL 8.5980 mL 17.1960 mL 42.9900 mL
5 mM 0.3439 mL 1.7196 mL 3.4392 mL 8.5980 mL
10 mM 0.1720 mL 0.8598 mL 1.7196 mL 4.2990 mL
15 mM 0.1146 mL 0.5732 mL 1.1464 mL 2.8660 mL
20 mM 0.0860 mL 0.4299 mL 0.8598 mL 2.1495 mL
25 mM 0.0688 mL 0.3439 mL 0.6878 mL 1.7196 mL
30 mM 0.0573 mL 0.2866 mL 0.5732 mL 1.4330 mL
40 mM 0.0430 mL 0.2150 mL 0.4299 mL 1.0748 mL
50 mM 0.0344 mL 0.1720 mL 0.3439 mL 0.8598 mL
60 mM 0.0287 mL 0.1433 mL 0.2866 mL 0.7165 mL
80 mM 0.0215 mL 0.1075 mL 0.2150 mL 0.5374 mL
DMSO 100 mM 0.0172 mL 0.0860 mL 0.1720 mL 0.4299 mL
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HKPerox-2 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

HKPerox-22245230-79-7HKPerox2HKPerox 2Fluorescent DyeReactive Oxygen SpeciesHydrogen peroxideH2O2O-methyl rhodolROSRNSpayne/dakin reactionRAW264.7Inhibitorinhibitorinhibit

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