Hydroxy naphthol blue disodium

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Hydroxy naphthol blue disodium, an azo dye, is a metal indicator for calcium and a colorimetric reagent for alkaline earth metal ions ( λmax = 650 nm). In the pH range between 12 and 13, the solution of the indicator is reddish pink in the presence of calcium ion and to deep blue in the presence of disodium EDTA.

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Hydroxy naphthol blue disodium Chemical Structure

CAS No. : 165660-27-5

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Description	Hydroxy naphthol blue disodium, an azo dye, is a metal indicator for calcium and a colorimetric reagent for alkaline earth metal ions ( λmax = 650 nm). In the pH range between 12 and 13, the solution of the indicator is reddish pink in the presence of calcium ion and to deep blue in the presence of disodium EDTA^[1].
Molecular Weight	598.49
Formula	C₂₀H₁₂N₂Na₂O₁₁S₃
CAS No.	165660-27-5
SMILES	O=C1C=C(S(=O)(O)=O)C2=CC=CC=C2/C1=N/NC3=C(O)C(S(=O)(O[Na])=O)=CC4=CC(S(=O)(O[Na])=O)=CC=C43
Shipping	Room temperature in continental US; may vary elsewhere.
Storage	Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation	Data Sheet (275 KB) Handling Instructions (2659 KB)
References	[1]. Motoki Goto, et al. Colorimetric detection of loop-mediated isothermal amplification reaction by using hydroxy naphthol blue. Biotechniques. 2009 Mar;46(3):167-72. [Content Brief]