IKKγ NBD Inhibitory Peptide TFA 

IKKγ NBD Inhibitory Peptide TFA is a highly specific inhibitor NF-κB inhibitor. IKKγ NBD Inhibitory Peptide TFA acts by disrupting the interaction between IKKγ/NEMO-binding domain (NBD) with IKKα and IKKβ, thus blocking TNF-α-induced NF-kB activation. IKKγ NBD Inhibitory Peptide TFA could significantly suppresses inflammation and ameliorate the cerebral ischemia-induced neurological deficits^[1][2][3].

In a canonical pathway, NF kappa B activation depends on the IKK complex activity, which is formed by three subunits (IKKκ and IKKβ and IKKγ/NEMO), thus the KKγ NBD Inhibitory Peptide TFA inhibits TNF-α-induced NF-kB activation^[2].
IKKγ NBD Inhibitory Peptide TFA (10 μM; 90 min) prevents Doxorubicin (HY-15142A)-induced (15 μM; 4 h) p65 phosphorylation in BT-474 cells^[2].
IKKγ NBD Inhibitory Peptide TFA (10 μM; 48 h) slightly inhibits BT-474 cell viability while it markedly enhances the effects on Doxorubicin^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

IKKγ NBD Inhibitory Peptide TFA (40 μg/4 μL; ICV; single dose; 2 h before MCAO) exhibits neuroprotective effect with reduction in DNA fragmentation, and its ischemic brain damage reduction mechanism be attributed to reduction in inflammation following ischemic injury in middle cerebral artery occlusion (MCAO) rats model^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

3807.32

C₁₇₂H₂₆₀N₄₉F₃O₄₄S₁

Solid

White to off-white

DRQIKIWFQNRRMKWKKTALDWSWLQTE

Room temperature in continental US; may vary elsewhere.

Sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

• [1]. Zhao J, et al. Development of novel NEMO-binding domain mimetics for inhibiting IKK/NF-κB activation. PLoS Biol. 2018 Jun 11;16(6):e2004663.  [Content Brief]

  [2]. Tapia MA, et al. Inhibition of the canonical IKK/NF kappa B pathway sensitizes human cancer cells to doxorubicin. Cell Cycle. 2007 Sep 15;6(18):2284-92.  [Content Brief]

  [3]. Desai A, et al. Neuroprotective potential of the NF-κB inhibitor peptide IKK-NBD in cerebral ischemia-reperfusion injury. Neurochem Int. 2010 Dec;57(8):876-83.  [Content Brief]