2. MCE Kits
  3. Molecular Biology
  4. PCR & qPCR
  5. Polymerase Chain Reaction (PCR)
  6. 2× High-Fidelity PCR Master Mix (with Dye)

2× High-Fidelity PCR Master Mix (with Dye) 

Cat. No.: HY-K0533A
Manual SDS

MCE 2× High-Fidelity PCR Master Mix (with Dye) is a new type of ready-to-use high-fidelity PCR amplification master mix.During the reaction, only template and primers need to be added to quickly and efficiently complete the PCR process, reducing the risk of contamination during operation.

2× High-Fidelity PCR Master Mix (with Dye)
Size Price Stock Quantity
Free Sample (100 μL)   Apply Now  
1 mL In-stock
5 mL Get quote
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  • Description

  • Storage

  • Components

  • Documentation

Description
& Advantages

MCE 2× High-Fidelity PCR Master Mix (with Dye) is a new type of ready-to-use high-fidelity PCR amplification master mix. This product contains a modified new rapid high-fidelity DNA polymerase, dNTPs, and an optimized buffer system. The DNA polymerase exhibits 5’→3’ DNA polymerase activity and 3’→5’ exonuclease activity. During the reaction, only template and primers need to be added to quickly and efficiently complete the PCR process, reducing the risk of contamination during operation.

The two monoclonal antibodies added to this product can inhibit the polymerase activity and 3’→5’ exonuclease activity at room temperature. Additionally, an extension factor has been included to enable the DNA polymerase’s ability to amplify long fragments, allowing for the amplification of target fragment up to 13 kb, making it suitable for complex templates. The amplified products have blunt ends.

This product contains bromophenol blue dye, allowing for direct electrophoresis detection of the PCR products without the need to add DNA loading buffer.
Storage

-20℃, 2 years

Avoid repetitive freeze-thaw cycles
Components
Components HY-K0533A-1 mL HY-K0533A-5 mL
2× High-Fidelity PCR Master Mix (with Dye) 1 mL 1 mL × 5
Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
2× High-Fidelity PCR Master Mix (with Dye)
Cat. No.:
HY-K0533A
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