1. MCE Kits
  2. Protein Biology
  3. Protein Purification
  4. Agarose Beads
  5. Ni-IDA Agarose

Ni-IDA Agarose 

Cat. No.: HY-K0238
Manual SDS

MCE Ni-IDA Agarose can be used for the detection and purification of His-tag proteins.

Ni-IDA Agarose
Size Price Stock
5 mL USD 85 Ask For Quote & Lead Time
10 mL USD 140 Ask For Quote & Lead Time
50 mL USD 522 Ask For Quote & Lead Time

* Please select Quantity before adding items.

  • Description

  • Storage

  • Components

  • Documentation

Description
& Advantages

MCE Ni-IDA Agarose is a highly cross-linked 4% agarose gel, synthesized through the chemically directed, high-density attachment of iminodiacetic acid (IDA). When chelated with Ni2+, it forms an extremely stable planar tetragonal structure, providing three ionic bonding sites. This configuration allows for the higher affinity purification of His-tag proteins from various expression sources such as E.coli, yeast, insect cells, and mammalian cells.

 

The specifications of the product correspond to the actual resin volume, with the resin content of 50%.

Storage

4℃, 2 years

Do not dry or freeze

Components
Components HY-K0238-5 mL HY-K0238-10 mL HY-K0238-50 mL
Ni-IDA Agarose 5 mL 10 mL 10 mL × 5
Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Ni-IDA Agarose
Cat. No.:
HY-K0238
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