Seamless DNA Assembly Ultra Kit 

Based on gene recombination, seamless cloning is a simple, efficient, and fast DNA cloning technology. A 15-20 bp sequence homologous to the end of the linearized vector is added to the 5’ end of the insert fragment primers. In the presence of enzymes, recombination can be easily achieved.

MCE Seamless DNA Assembly Ultra Kit is a next-generation recombinant cloning kit that allows the recombination of single or multiple DNA fragments in a single reaction.

—Features of MCE Seamless DNA Assembly Ultra Kit

1. Fast: Single fragment recombination can be completed in as little as 5 min, with a positive rate of over 95%. —

—2. High Fidelity: Utilizes an upgraded high-fidelity Taq DNA Ligase, significantly increasing the success rate of seamless cloning compared to regular Taq DNA Ligase.

—3. High Transformation Efficiency: The mix includes a transformation enhancer, greatly improving transformation efficiency.

—4. Optimized Buffer: The optimized buffer components provide greater stability and significantly enhanced antioxidant performance.

-20℃, 2 years

Avoid repeated freeze-thaw cycles