2. MCE Kits
  3. Molecular Biology
  4. Restriction Endonuclease
  5. Sph I

Sph I 

Cat. No.: HY-KE7040
Manual SDS

MCE Sph I is a restriction enzyme for rapid DNA digestion, including plasmid, genomic DNA as well as PCR products. Isoschizomers: Pae I.

Sph I
Size Price Stock Quantity
50T USD 31 In-stock
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  • Description

  • Storage

  • Components

  • Documentation

Description
& Advantages

•    Sph I is 100% active in 10× Buffer and 10× Color Buffer. 10× Color Buffer includes a density reagent along with red and yellow tracking dyes that allow for direct loading of the reaction mixtures on a gel. The red dye of the Color Buffer migrates with 2500 bp DNA fragments in a 1% agarose gel and the yellow dye of the Color Buffer migrates faster than 10 bp DNA fragments in a 1% agarose gel.

 

•   Cleavage site:

 

3’…G  C  A  T  G▼C…5’


5’…C▲G  T  A  C  G…3’
Storage

-20°C, 2 years.
Components
Components HY-KE7040-50T
Sph I 50 μL
10× Buffer 1 mL
10× Color Buffer 1 mL
Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Sph I
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HY-KE7040
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