Taq DNA Ligase

Cat. No.: HY-KE8009: FAQs
Manual SDS

This enzyme is a DNA ligase cloned from Thermus aquaticus HB8. It is expressed in E. coli using genetic recombination technology and purified and isolated multiple times.

Size	Price	Stock
1000 U	USD 50	Ask For Quote & Lead Time

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Description
Storage
Components
Documentation

Description
& Advantages

Taq DNA ligase catalyzes the formation of phosphodiester bonds, connecting the 5´-phosphate termini and 3´-hydroxy termini of two conforming oligonucleotide strands hybridized to the same complementary target DNA strand through phosphodiester bonds. This ligation reaction can only occur when the two oligonucleotide strands are completely paired with the complementary target DNA and there is no gap between the two oligonucleotide strands. Therefore, it can be used to detect single base substitutions. Taq DNA ligase is active in the range of 45 to 65°C.

Storage

-20ºC, 1 years

Components

Components	HY-KE8009-1000 U
Taq DNA Ligase (40 U/μL)	25 μL
10×Taq Ligase Reaction Buffer	500 μL

Documentation

Manual (2926 KB) SDS (10353 KB)

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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