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  2. Protein Biology
  3. Protein Sample Preparation
  4. Washing Buffer
  5. TBS Powder (1 L of 1×)

TBS Powder (1 L of 1×) 

Cat. No.: HY-K1026
Manual SDS

MCE TBS Powder (1 L of 1×) consists of NaCl, KCl and Tris, and is widely used as washing buffer in immunocytochemistry, FISH or ELISA, and can also be used for antibody dilution and preparation of blocking solution.

TBS Powder (1 L of 1×)
Size Price Stock Quantity
Free Sample (2 pouches)   Apply Now  
20 pouches USD 62 In-stock
100 pouches USD 185 In-stock

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  • Description

  • Storage

  • Protocol

  • Components

  • Documentation

Description
& Advantages

TBS buffer is widely used as washing buffer in immunocytochemistry, FISH or ELISA, and can also be used for antibody dilution and preparation of blocking solution.

MCE TBS Powder (1 L of 1×) consists of NaCl, KCl and Tris. Each pouch can be diluted to 1 L 1× TBS buffer, in which the concentration of NaCl is 137 mM, the concentration of KCl is 2.7 mM and the concentration of Tris is 25 mM. This product provides a convenient way to make TBS buffer and eliminates the need to weigh and mix individual components.

Storage

Store at room temperature for 3 years.

Protocol

1. Add 1 pouch of TBS Powder into the cleaned beaker, dissolve with 600 mL distilled water under a magnetic stirrer.

2. Add distilled water to the solution in step 1 until the total volume is 1 L. The final solution is 1× TBS buffer.

Note: The pH of the 1× TBS buffer is 7.4 ± 0.15 @ 25°C.

Components
Components HY-K1026-20 pouches HY-K1026-100 pouches
TBS Powder (1 L of 1×) 20 pouches 5 × 20 pouches
Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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TBS Powder (1 L of 1×)
Cat. No.:
HY-K1026
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