JAK-2/3-IN-3

Cat. No.: HY-151285: FAQs
Data Sheet Handling Instructions

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JAK-2-/3-IN-3 (compound ST4j) is a potent JAK2/3 inhibitor with IC₅₀s of 13.00 and 14.86 nM for JAK2 and JAK3, respectively. JAK-2-/3-IN-3 inhibits autophosphorylation of JAK2 and induces apoptosis in a dose- and time-dependent manner. JAK-2-/3-IN-3 can be used in studies of lymph derived diseases and leukemia.

For research use only. We do not sell to patients.

JAK-2/3-IN-3 Chemical Structure

CAS No. : 2242031-31-6

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JAK1 JAK2 JAK3 Tyk2 JAK

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[1]

JAK2

13.00 nM (IC₅₀)

JAK3

14.86 nM (IC₅₀)

In Vitro

JAK-2/3-IN-3 (0-100 μM; 72 h) specifically inhibits human erythroleukemia cells with low toxicity to the normal cells^[1].
JAK-2/3-IN-3 inhibits human erythroleukemia TF1 cell growth via the JAK2/STAT5 signaling pathway^[1].
JAK-2/3-IN-3 (15.53 μM; 24, 48, 72 h) induces cytotoxicity via apoptosis in a time-dependent manner in TF1 cells^[1].
JAK-2/3-IN-3 (IC₂₅, IC₅₀, IC₇₅; 24 h) induces cytotoxicity via apoptosis in a dose-dependent manner in TF1 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis^[1]

Cell Line:	TF1 cells
Concentration:	15.53 μM
Incubation Time:	24, 48, 72 h
Result:	Increased cells apoptotic by ∼20%, ∼40% and ∼70%, after 24-, 48- and 72-h incubations, respectively. Increased cells apoptotic by ∼15%, 20%, and 30% with concentration of IC₂₅, IC₅₀ and IC₇₅, respectively.

Cell Cytotoxicity Assay^[1]

Cell Line:	TF1, HEL, Vero and HepG2 cells
Concentration:	0-100 μM
Incubation Time:	72 h
Result:	Inhibited growth of TF1 and HEL cells, with IC₅₀s of 15.53 and 17.90 μM. Showed low toxic to normal Vero cells and HepG2 cells (IC₅₀ of >100 μM in Vero cells and of >50 μM in HepG2 cells).

Molecular Weight

325.15

Formula

C₁₃H₁₀Cl₂N₄O₂

CAS No.

2242031-31-6

SMILES

O=C1NC2=C(N=C1C3=C(N(C)N=C3C)O)C=C(Cl)C(Cl)=C2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Sanachai K, et al. Discovery of JAK2/3 Inhibitors from Quinoxalinone-Containing Compounds. ACS Omega, 2022.

JAK-2/3-IN-3 Related Classifications

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)	×	Volume _(start)	=	Concentration _(final)	×	Volume _(final)
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

JAK-2/3-IN-32242031-31-6ApoptosisJAKJanus kinaseTF1HELVeroHepG2lymph derived diseasesleukemiacancerInhibitorinhibitorinhibit

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