2. Membrane Transporter/Ion Channel
  3. Piezo Channel
  4. Jedi2

Jedi2 is a Piezo1 activator, but not a specific Piezo2 activator. Jedi2 binds to the mouse Piezo1 proteins with a Kd of 2770??μM.

For research use only. We do not sell to patients.

Jedi2 Chemical Structure

Jedi2 Chemical Structure

CAS No. : 651005-90-2

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 105 In-stock
Solution
10 mM * 1 mL in DMSO USD 105 In-stock
Solid
5 mg USD 95 In-stock
10 mg USD 150 In-stock
50 mg USD 590 In-stock
100 mg   Get quote  
200 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Jedi2 Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Jedi2

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Jedi2 is a Piezo1 activator, but not a specific Piezo2 activator. Jedi2 binds to the mouse Piezo1 proteins with a Kd of 2770??μM[1].

In Vitro

Jedi2 evokes Piezo1-mediated Ca2+ influx. Jedi2 (200 μM) specifically elicits dose-dependent responses in mPiezo1-transfected cells, but not in mPiezo2- or vector-transfected cells [1].
Jedi2 (1 mM) causes an increase in Ca2+ in mCherry-positive HEK293T cells transfected with the mPiezo1-mCherry construct, which also responded to Yoda1 (30 μM) [1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Jedi2 Related Antibodies
Molecular Weight

208.23

Formula

C10H8O3S
CAS No.
Appearance

Solid

Color

Light brown to brown

SMILES

O=C(C1=C(C)OC(C2=CC=CS2)=C1)O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (240.12 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.8024 mL 24.0119 mL 48.0238 mL
5 mM 0.9605 mL 4.8024 mL 9.6048 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: 2.5 mg/mL (12.01 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.93%

COA (250 KB) RP-HPLC (249 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.8024 mL 24.0119 mL 48.0238 mL 120.0596 mL
5 mM 0.9605 mL 4.8024 mL 9.6048 mL 24.0119 mL
10 mM 0.4802 mL 2.4012 mL 4.8024 mL 12.0060 mL
15 mM 0.3202 mL 1.6008 mL 3.2016 mL 8.0040 mL
20 mM 0.2401 mL 1.2006 mL 2.4012 mL 6.0030 mL
25 mM 0.1921 mL 0.9605 mL 1.9210 mL 4.8024 mL
30 mM 0.1601 mL 0.8004 mL 1.6008 mL 4.0020 mL
40 mM 0.1201 mL 0.6003 mL 1.2006 mL 3.0015 mL
50 mM 0.0960 mL 0.4802 mL 0.9605 mL 2.4012 mL
60 mM 0.0800 mL 0.4002 mL 0.8004 mL 2.0010 mL
80 mM 0.0600 mL 0.3001 mL 0.6003 mL 1.5007 mL
100 mM 0.0480 mL 0.2401 mL 0.4802 mL 1.2006 mL
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Jedi2 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Jedi2651005-90-2Jedi 2Jedi-2Piezo ChannelMechanosensitivePiezoPiezo1Piezo2channelfunctionInhibitorinhibitorinhibit

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